Abstract: Lycium ruthenicum Murr. is a plant that has the concomitant function of medicine and edible in Tibet. In this paper，based on a large number of related literature，the author summarized the chemical constituents、pharmacological activity and cultivation techniques of Lycium ruthenicum Murr.，examined the current status of Lycium ruthenicum Murr. development and application in food，medicine and cosmetic industry. Aiming at providing reference for further study and rational use of Lycium ruthenicum Murr..

Abstract:The effect of refrigeration on dough fermentation property and bread quality in terms of specific volume，texture and flavor were studied. Different refrigeration conditions included refrigeration temperature，storage period and pre-fermentation. The results revealed that lower refrigeration temperature and less pre-fermentation could increase the duration of yeast gas production. Meanwhile，longer pre-fermentation time and higher refrigeration temperature would shorten the time to reach the largest specific volume. A better texture was also obtained at longer pre-fermentation time and higher refrigeration temperature. In addition，GC-MS results showed that the refrigeration could increase the content of bread flavor，and the volatiles content would increase with the growth in storage time.

Abstract:In order to overcome the defect of low catalytic efficiency of conventional cross-linked enzyme aggregates（CLEAs） toward macromolecular substrate，porous CLEAs（p-CLEAs） was applied in the degradation of chitosan to produce bioactive chitosan oligomers. The reducing sugar concentration was 85% of that catalyzed by free papain，which is 4 times higher than the conventional CLEAs under the same condition. Molecular weight and polydispersity index displayed the particular properties in the degradation of chitosan catalyzed by p-CLEAs compared with free papain. By controlling the degradation time，67.5%（wt） of the oligomer product（5~10 kD） were obtained through ultrafiltration membrane separation. Staphylococcus aureus and Escherichia coli can be totally suppressed when the concentration of oligomers（5~10 kD） was 0.275mg/mL.

Abstract:Nε-（carboxymethyl） lysine（CML） concentrations in instant noodles of 20 brands were determinated using enzyme-linked immunosorbent assay（ELISA），then developmental and reproductive toxicity of CML were evaluated in vitro. Concentrations of CML in free form and bind form were 0.39~0.80 mg/kg and 19.42~34.99 mg/kg，respectively. No significant effect of CML was found on cell viability（CML≤1 mmol/L） and progesterone secretion（CML≤2 mmol/L） of R2C cells treated with CML for 24 h. Moreover，CML of concentration lower than 4 mmol/L has no significant effect on 3T3 cells treated with CML for 10 days，and CML of concentration lower than 0.5 mmol/L has no significant effect on D3 cells treated with CML for 10 days. According to the concentrations of CML detected in the present study，CML would not inhibit the progesterone secretion of R2C cells or cell viability of R2C，3T3，and D3 cells with regular intake of instant noodles. Thus，regular intake of instant noodles would not cause developmental or male reproductive toxicity induced by CML.

Abstract:The spring dextrin were prepared by enzymatic hydrolysis of waxy maize starch with pullulanase alone，and the process parameters were optimized by one-factor-at-a-time method and response surface methodology. The optimal results were observed at conditions of enzyme dosage 420 U/g，hydrolysis for 6 h at 53.32 ℃，pH 4.96，predicted using mathematical predictive model. Under these conditions，the spring dextrin purity was 99.2%. The chain-length distribution of the prepared spring dextrin were also evaluated. Results showed that the proportion of spring dextrin with DP＜60 were 95.5%，and the relative contents of spring dextrin with DP6~12，DP13~24，DP25~36 and DP37~60 were 24.40%，45.08%，19.60% and 10.92%，respectively.

Abstract:High-fluorine is the potential restriction factor in application of Antarctic Krill. The study explores the potential of iron-impregnated bamboo charcoal for the removal of fluoride from Antarctic Krill hydrolyzate. Batch experiments were conducted to establish the optimal conditions like particle size，pH，contact time，temperature and dose of the adsorbent. The optimal conditions were as follows：absorbent additive dosage 0.01 g/mL，contact time 60 min，temperature 40 ℃，particle size 40-60 mesh，initial pH. Fluoride level of hydrolyzate can be reduced to 5.1 mg/L under the optimum condition when initial fluoride concentration of 18.7 mg/L is employed. Removal efficiency was promoted to 73% using modified bamboo charcoal ，which is better than unmodified one.

Abstract:Guar gum was hydrolyzed in the best hydrolysis conditions and its hydrolysate was analyzed by HPGFC and HPLC. The grafted products of guar gum hydrolysate and egg white were obtained at 60 ℃，relative humidity 79% in different weight ratio，pH value and reaction time，and their gel strength and water holding capacity were studied. Also，amino acid analysis，SDS-PAGE and SEM were use to characterize gel properties of the grafted product. The results showed that the main components of guar gum hydrolysate were manno-oligosaccharides and monosaccharide was hardly existed. To get the best gel strength and water holding capacity，the optimum reaction conditions to prepare the grafted product were studied，that were weight ratio 0.5%，pH value 9.0 and reaction time 4 d. In that condition，the modified protein powder had high gel strength in pH 4~10，and had good storage stability. As the extension of the modification time，the contents of free amino and amino acids of the modified protein powder were decreased. SDS-PAGE analysis showed that in the process of grafting reaction，large molecular weight compounds were produced between the guar gum hydrolysate and egg white. Network structures in the gel of the modified protein powder were more uniform and compact compared to the original protein powder，which was observed under SEM. In general，the grafting reaction between guar gum hydrolysate and egg white can effectively improve the gel properties of egg white protein.

Abstract:To overcome the time-consuming screening process，mammalian cells expressing recombinant therapeutic protein were selected by flow cytometry and method of cell purity analysis was established. Transfected CHO cells were sorted by flow cytometer and high expression sub-clone was screened by dot blot and western blot. CHO cells after transfecting with rFVII expression vector were divided by flow cytometry to 96 micro-plates and recombinant coagulation factor VII expression was analyzed by dot blot and western blot. High rFVII expression clone，CHO-rFVII-1，was obtained and the purity of CHO-rFVII-1 was 99.9 %. Moreover，CHO-rFVII-1 had similar viable cell density and rFVII expression compared to CHO-rFVII，which was obtained by traditional screening method. Screening high expression clones by flow cytometry was a rapid and high-throughput method and will be beneficial to screen high recombinant therapeutic protein expression clone in other mammalian cells.

Abstract:According to the GB 5413.16 2010，GB/T 5009.211 2008 and Biostest different methods，On the same folic acid content in dairy products and for folic acid determination of standard addition recovery experiment was carried out at the same time. Results show that according to GB 5413.16 2010，in the preparation of standard test tube，using two concentration with phosphate buffer containing ascorbic acid dilution folic acid standard working liquid，liquid sample tests also diluted by ascorbic acid phosphate buffer，measured folic acid content is 40.45 μg/100 g，the recovery was 71%. But GB/T 5009.211 2008，using only a concentration of diluted folic acid standard working liquid，liquid sample tests also get diluted by water，measured the same samples of folic acid content is 70.5 μg/100 g，the recovery was 92.6%. Using Biostest folic acid detection kit measured the same samples of folic acid content is 73.71 μg/100 g，the recovery was 99%. Conclusion is not using ascorbic acid in phosphate buffer but diluted standard working liquid and liquid sample tests，test infant dairy folic acid content，the standard addition recovery rate is higher，test results are acceptable.

Abstract:A Rhizobium sp. capable of producing curdlan was isolated from soil. The polysaccharide product was identified to be curdlan by testing the thermal property，molecule composition，infrared spectrum and molecule weight. Furthermore，the orthogonal optimization experiment was conducted to optimize the curdlan fermentation medium，and the best medium composition was 50 g/L glucose，2 g/L（NH4）2HPO4，1.5 g/L KH2PO4，1.25 g/L MgSO4，0.05 g/L FeSO4·7H2O，0.02 g/L MnSO4，0.01 g/L CoCl2·6H2O，0.01 g/L ZnCl2. Consequently，the curdlan production and glucose conversion rate were enhanced by 29.2% and 5.4% compared with those before optimization.

Abstract:The inhibitory effects of α-amylase，α-glycosidase，pancreatic lipase，pancreatic cholesterol esterase and cholesterol micelle solubility were the evaluation index and then screened 9 functional active components factors. Tea polyphenols，gingko flavonoids，gynostemma saponins，peony seed oil and momordica charantia extracts were the final formula of the active ingredients. Inhibitory rate of α-amylase and cholesterol esterase were used as evaluation index to determin the main components of the formula by the method of orthogonal t value analysis and weighted synthesis. The main compositions were tea polyphenols，gingko flavonoids and peony seed oil，the adjuvant were gynostemma saponins and momordica charantia extracts.

Abstract:In order to obtain high quality bean worm of vacuum freeze-drying，the influence of blanching temperature and drying chamber pressure was studied in this paper by five indexes，that is freeze-dried success rate，drying time，protein retention rate，coloration change and the rehydration rate，are took to measure the quality of vacuum freeze-drying processes. The eutectic point of bean worm is -19.9 ℃，which is determined with differential scanning calorimetry. So the pre-freezing temperature of beam worm is -28 ℃. The result indicated that the success rate could reach as highly as 97%，while the blanching temperature was 60 ℃. The protein retention rate reached the highest when blanching temperature was 50 ℃ and the lowest at 80 ℃. When the drying chamber pressure decreases，the retention rate of the protein has a decreasing tendency. Drying chamber pressure had less influence on the color change of beam worm. The drying rate was the best when the drying chamber pressure was 57 Pa with the 60 ℃ blanching temperature and the rehydration rate of bean worm can reach the highest.

Abstract:The polysaccharides from Choerospondias axillaries fruit was extracted by distilled water，and the extraction parameters were optimized using L9（34） orthogonal test with extraction temperature，extraction time，solid-liquid ratio and extraction times as main effect factors. The results indicated that the influence order of them was extraction temperatures，extraction time，solid-liquid ratio and extraction times，respectively. And when the polysaccharides was extracted at 100 ℃ for one time，with a extraction time of 5 h and a solid-liquid ratio of 1∶40（g/mL），the yield of polysaccharides was up to 21.79%.

Abstract:To study the effect of two modified konjac glucomannan on the growth performance，the liver based ingredients，serum biochemical indicators and lipid metabolism-related gene expression of Schizothorax prenanti. Schizothorax prenanti serum high-density lipoprotein cholesterol（HDL-C），low-density lipoprotein cholesterol（LDL-C），total cholesterol（T-CHO） and triglyceride（TG） were determined based on enzyme-linked immunosorbent assay（ELISA）.The number of lipoprotein lipase（lpl），hepatic lipase（hl） and fatty acid synthetase（fas） in the liver were quantified by real-time quantitative PCR. The results showed as follows：modified konjac glucomannan type，adding dose and the interaction level had no significant influence on Schizothorax prenanti growth，grossness degree. In konjac glucomannan sulfate（OKGMS） modification conditions，with the increase of adding dose liver fat，serum LDL - C and T - CHO and TG content decreased significantly，when adding 1.6% OKGMS reached the lowest；In Acid oxidation of konjac glucomannan（AOKGM），with the increase of adding dose serum LDL - C，T - CHO and TG content is increased after lower first，when adding 0.8% AOKGM reached the lowest，all groups significant differences with the control group. Compared with the basic group，adding 1.6% OKGMS，0.8% AOKGM significantly increase serum HDL-C level，liver LPL mRNA expression quantity increased by 7.7 and 2.7 times respectively.