Abstract:Fuel ethanol is developed to resolve the problems of fossil energy draining and vehicle air pollutant. This cleaner fuel is also considered as inevitable choice to keep energy strategy safe and develop low carbon economy. Therefore，worldwide ethanol production has increased sharply for last decades. However，wastewater handling problem become increasingly severe at the same time. It is very important to improve the wastewater treatment technology to achieve sustainable development of bioethanol industry. The domestic and foreign researchers developed many ethanol wastewater treatment technologies，like evaporation，reuse，single cell protein/biologics production，farmland irrigation，anaerobic digestion，integrated ethanol-methane fermentation process and other technologies. This papersystematicallysummarized these technologies，elaborating theories，characteristic，advantages and disadvantages of these technologies in order to offer references for the further researches on the ethanol wastewater treatment.

Abstract:5-aminolevulinic acid（ALA），a naturally occurring non-protein amino acid，has been widely applied in the industry of medicine and agriculture. To construct a recombinant Saccharomyces cerevisiae for production of ALA，genes hem1 cloned from S. cerevisiae and hemA cloned from Rhodobacter spheroides 2.4.1 encoded ALA synthase of C4 pathway was overexpressed，respectively. Meanwhile，glutamyl-tRNA reductase（encoded by hemA） and glutamate-1-semialdehyde aminotransferase（encoded by hemL），the key enzymes of C5 pathway from Escherichia coli were also overexpressed. The result demonstrated that overexpression of hem1 was more beneficial to the production of ALA，327.6 mg/L. Moreover，genes hem1，hemA and hemL were co-overexpressed，resulting recombinant strain IN-PY1AL. The production of ALA was 525.8 mg/L with addition of precursor glycine and succinic acid. This realized the biosynthsis of ALA in S. cerevisiae.

Abstract:In this study，an alcohol dehydrogenase KpADH from Kluyveromyces polysporus was identified by genome mining strategy. This newly mined KpADH belongs to extended SDRs subfamily，and catalyze the asymmetric reduction of diaryl ketones into chiral diaryl alcohols. KpADH also displays substrate-coupled NADPH recycling ability by oxidation of cosubstrate isopropanol. The recombinant KpADH was purified by Ni-NTA affinity chromatography，and the enzymatic properties of the purified KpADH were characterized. The optimum pH of oxidizing and reducing activity is 9.5 and 5.5 respectively. The Km and Vmax of KpADH towards CPMK are 0.5 mmol/L and 25 μmol/（min·mg） respectively，while the Km and Vmax of KpADH towards isopropanol are 6.36 mmol/L and 21.37 μmol/（min·mg） respectively. KpADH shows higher catalytic activity on ketone esters and 2，3-butanediol. Within 10 h，100 mmol/L CPMK was asymmetrically reduced into（R）-CPMA by recombinant E. coli BL21/pET28a-kpadh cells，giving >99% conversion，82% ee and 88.7% molar isolation yield. This study provides useful guidance for the application of this newly identified KpADH in the preparation of chiral diaryl alcohols.

Abstract:In this study，the endophyte fungi of lawender was isolated and identified.18 and 23 endophytic fungi were isolated from planting lavender and wild lavender. The strains were cultured by solid-state fermentation and to extract the fermentation culture products. Wild lavender extracted a strain endophytic fungi of phenolic acids by using the method of chemical reaction. And the strains were identified by the method of molecular biology. Solid-state fermentation extract was identified primarily. The diversity of endophytic fungi of lavender is reflected by the results of the study and the solid fermentation by microorganisms to produce phenolic acid material provided theoretical and experimental basis.

Abstract:The effects of pressure，temperature，pH and holding time on Candida rugosalipase activity were studied and the recovery of the enzyme was analyzed. We also investigated the changes of kinetics parameters of lipase under different temperature and pressure. The results showed that lipase activity was the highest under 200 MPa and almost 1.76 times of atmospheric pressure. Under this pressure，the optimum temperature and pH for lipase were 40 ℃and pH 7.5，respectively；the processing time was 10 min；the change of Km at 40 ℃ is not as obvious as 50 ℃；meanwhile the Vmax，Kcat and Kcat/Km increased significantly with the elevated pressure.

Abstract:Pipecolic acid is an important precμrsor for piperidine ring derivatives. A lysine cyclodeaminase was used to catalyze L-lysine for L-pipecolic acid synthesis. To accelerate the catalytic rate，a PDMS microchip was designed and fabricated. Effects of microchannel size，microchannel length and liquid flow rate on catalytic rate were studied. The optimized conditions in microreactor were：microchannel size 200 μm；microchannel length 124.8 cm；and liquid flow rate 100 μm/min. Under the optimized conditions，a pipecolic acid titer of 2.22 g/L was obtained after 44 h reaction，the reaction time reduced 1/3 in comparison with the reaction in shaking tube.

Abstract:This study was to develop fermented milk with low alcohol by selecting the strain of lactic acid bacteria and yeasts from koumiss of Xinjiang Autonomous Region. The fermentation of skim milk by lactic acid bacteria and yeasts was investigated. Mixed fermentation conditions were optimized by the single-factor test and orthogonal experiment，after which the physicochemical properties of the fermented milk were assayed. Conclusions are as follows：the selected lactic acid bacterium was Lactobacillus crustorum and the yeast was Trichosporon asahii. The process parameters are as follows：fermentation temperature was 37 ℃，fermentation time was 14 h，inoculation amount was 4%，lactic acid bacteria∶yeast = 2∶3. The physicochemical properties of the fermented milk with low alcohol product were：pH（5.54±0.035），titration acidity（40.9±0.608） °T，ethanol（0.266±0.047） %，total acid（3.274±0.197） g/L，amino acid nitrogen（0.372±0.0285） g/L，retention ability（31.28±0.0187） %，reducing sugar（76.93±3.07） mg/mL.

Abstract:Ultrasound and complex enzymatic hydrolysis can be used to enhance utilization level of raw material and ensure sensory quality of fruit wine under fermentation process. In this study，effects of different ultrasound-enzymatic processing on quality and aroma components of ginger wine were investigated. Alcoholic strength，fermentable sugar content，total sugar content，titratable acid content and free amino acid content of ginger wines under different processing were compared，and aroma composition of various ginger wine samples were analyzed using GC-MS. The results indicated that alcoholic strength of simultaneous processing wine was increased by 9.66% and 7.61% compared with ultrasonic processing wine and enzymatic processing wine. Similarly，fermentable sugar content was increased by 43.68% and 15.19%，total sugar content was increased by 46.29% and 0.52%，titratable acid content was increased by 49.70% and 18.40%，free amino acid content was increased by 18.17% and 18.06%. The results of aroma composition analysis indicated that ginger wines under different ultrasound-enzymatic processing were mainly composed of alcohols，esters，acids，a few of phenols and alkanes，and the relative content of alcohol compounds was the highest. Aroma substances generated from simultaneous processing wine were most，and 51 compounds were identified. One of the highest relative content of these compounds was phenethyl alcohol，accounting for 19.6%. Relative contents of butyrolactone，hexanal，2-methylcaproic acid and 6-gingerol were also significantly higher than the other processing wines，accounting for 5.42%，5.09%，5.01% and 10.5%.

Abstract:In order to enhance the production of RGD-TRAIL by recombinant E.coli，response surface methodology was applied to optimize the fermentation conditions. Based on the single factor experiments，the inoculation volume，pH value，the induction temperature and induction time were optimized by response surface optimization with 4 factors 3 levels using Box-Behnken experimental design. The optimum conditions for production of RGD-TRAILwere determined as follows：inoculation volume of 7.88%，pH 7.02，the induction time of 10.25 h，induction temperature of 22 ℃. Under the optimal fermentation conditions，the expression of RGD-TRAIL was 17.27%，which was close to the model prediction expression and 40.06% higher than that before optimization.

Abstract:The DPE gene from Clostridium bolteae ATCC BAA-613 was cloned and expressed in Bacillus subtilis WB800. We inserted a P43 promoter directly upstream the DPE geneby virtue of overlap extension PCR，which forming P43-Cb-dpe，and then ligated it into pMA5 shuttle vector thus constructing the pMA5-P43-Cb-dpe expression vector. The recombinant plasmid was imported into Bacillus subtilis WB800 for expression. Compared with single promoter expression system，pMA5-P43-Cb-dpe has a palpable promotion of expression level. The expressed recombinant enzyme was purified andits enzymatic properties were studied. The optimum temperature and pH for recombinant enzyme were 55 ℃ and pH 7.0，respectively. It was highly stable at 30～40 ℃ and pH 6.5～7.5. Co2+ and Mn2+ can significantly enhance enzyme activity. The recombinant enzyme's affinity for D-psicose was high than D-frutcose. Meanwhile，in terms of the kinetic parameters，the catalytic efficiency Kcat/Km is greater when use D-psicose as substrate.

Abstract:In order to optimize the processing technology of yak yogurt，sterilization conditions of yak milk，starter cultures，fermentation time，post-fermentation time were researched based on standardization analysis. The results showed that yak milk sterilization for 10 min at 90 ℃，starter culture with 0.15%，fermentation at 43±1 ℃，and post-fermentation for 24 h at 4 ℃，yak yogurt had good quality.

Abstract:Effects of various parameters on cellobiose dehydrogenase（CDH） production by Pycnoporus sanguineus SYBC-L10. CDH was induced by cellulosic，and microcrystalline cellulose with high crystallinity supported the maximum CDH production. Though the optimization of fermentation medium composition，microcrystalline cellulose and glucose as the complex carbon source were add to the culture，the production of CDH reached 78±5.94 U/L，with 2 folds higher than that before optimization（39±4.78 U/L）.Though adding the soybean meal and（NH4）2HPO4 to the culture，the production of CDH reached 702±25.68 U/L，with 17 folds higher than that before optimization. Selecting the optimum carbon sources and nitrogen sources to get the optimum liquid medium recipe by the orthogonal test in four factors and four levers，The production of CDH reached 1245±35.78 U/L. The optimum fermentation medium consisted：microcrystalline cellulose 7 g/L，glucose 5 g/L，soybean meal 6 g/L，（NH4）2HPO4 2 g/L. The production of CDH reached 1 245±35.78 U/L.

Abstract:This study constructed a secretive recombinant plasmid pMG36e-usp45-egl3 and E. coli DH5α/pMG36e-usp45-egl3 with a fusion gene usp45-egl3 connected by the secreted protein gene sequence of usp45（81 bp） from Lactococcus lactis and the coded mature-protein gene sequence of egl3（657 bp） from Trichoderma reesei by the method of overlapping extension PCR. After analyzing the expressive effect of recombinant Escherichia coli and evaluate the capability of secreted β-1，4-endoglucanase degrading fiber. The results showed that the recombinant E.coli DH5α/ pMG36e-usp45-egl3 could secrete β-1，4-endoglucanase with activity of 226 mU/mL after culture 14 h and effectively degrade sodium carboxymethylcellulose. The rate of β-1，4-endoglucanase degrading sodium carboxymethylcellulose into reducing-sugar was 2.35 mg/（h·mL）.

Abstract:Seriflux is the by-product of the Chinese rice wine production，which has rich in nutrient with low pH. Direct wastewater treatment process of seriflux caused great economic burden because of the high COD and failed to utilize nutrient. For recycling the seriflux，108 yeast strains were isolated from the enviroment. According to the biomass and pigment production of the red yeast cultured in pH 3.5 medium，5 red yeasts with better characteristics were selected. Further study on cell population，pigment content and COD degradation rate of the yeast inoculated in seriflux，strain R-70 was screened as the suitable yeast. According to the morphological and physiological characteristics，combined with 26S rDNA sequence analysis result，R-70 was identified as Sporidiobolus pararoseus. Fermentation experiments of R-70 in seriflux showed that pH and the amount of ammonia nitrogen increased with during the culture process. The number of yeasts cells reached the maximum 7.8×107/mL at 36 h，and the pigment content reached a higher value 5.26 mg/L at 44 h. Meanwhile the COD decreased to the minimum at 44 h，the removal rate of COD was up to 91.12%. Sporidiobolus pararoseus R-70 is suitable for seriflux recycling with good performance.

Abstract:This paper studies the effect on hyperlipemia mice with the Prinsepia utilis tea which were be fermented by the "golden flower fungus". Hyperlipidemia mice were induced by intragastric administration of high fat forage，meanwhile，other mice were fed by standard normal diet as blank control group for 4 weeks. Then the heperlipidemia mice were randomly divided into 6 groups：the model group，the 3 doses of Prinsepia utilis fermented tea treatment groups（2，6，9 g/kg），the Prinsepia utilis dry tea group and the positive control group（3 g/kg）. Respectively，each of the groups was treated by intragastric process for 28 days，then gained the serum by separating the heart blood，and detected the total cholesterol（TC），triglyceride（TG），high density lipoprotein cholesterol（HDL-C），low-density lipoprotein cholesterol（LDL-C），apolipoprotein AI（Apo-AI），apolipoprotein B（Apo-B） and lipoprotein a（LP-a）. Calculate the liver index，then make the liver pathology section. Compared the 3 fermented tea treatment groups with the model group，TC，TG，LDL-C and the ratio of Apo-B to Apo-Ai was significantly lower（p<0.05）. It has reached a very significant（p<0.01），the HDL-C and the liver index in the 3 doses fermented tea group，and TC，TG and LDL-C in the high dose group，meanwhile，curbing excessive increase of HDL-C in the treatment groups. Compared with the Prinsepia utilis dry tea group，it was not significant amount of weight gain in the medium and low dose fermented tea treatment groups，but significantly reduced in the low dose group（p<0.05）. The Prinsepia utilis tea fermented by the "golden flower fungus"，can effectively control the hyperlipidemia disease，decrease the weight gain of the mice，and significantly reduce or inhibit the biochemical indicators in hyperlipidemia mice，so with the help of golden flower fungus，biological indicator，especially，lipid-lowering can be appeared better efficacy than those without fermented tea.

Abstract:The purification，components analysis and hypolipidemic activity of a polysaccharide from Camellia oleifera Abel. seeds（CPSI） were studied. The crude polysaccharides were extracted from the seeds of Camellia oleifera Abel. using deionized water. The Camellia oleifera seeds polysaccharides（CPS） were further separated and purified by anion exchange chromatograph on DE-52，Sephadex G-100 column and affinity chromatography. The monosaccharide composition in the Camellia oleifera seed polysaccharide Ⅰ was determined by capillary electrophoresis.The potential mechanisms underlying lipid-lowering affect of CPSI were examined，specifically alterations in levels of the total cholesterol（T-CHO），High-density lipoprotein cholesterol（HDL-C），low-density lipoprotein cholesterol（LDL-C），3-hydroxy-3-methyl glutaryl coenzyme A reductase（HMG-CoA） and coprosterol，using a mice model of hypercholesterolemia. CPSⅠwas conjugated polysaccharide which has many phosphate groups. The polysaccharide contained xylose and galactose，and the mole ratio of galactose and xylose was 4.6∶5.57 in the hydrolytic solution of CPSⅠ.Serum T-CHO，LDL-C levels in mice treated with CPSI declined significantly when compared to hyperlipid control group，and coprosterol of mice in the groups treated with CPSI was higher than that in hyperlipid control group.Serum HDL-cholesterol and HMG-CoA activity，however，was unchanged.These results indicate that CPSI is useful for the prevention of hyperlipidemi in mice and it may therefore play a valuable role in reducing cardiovascular risk.