Abstract:As a kind of chronic metabolic diseases，diabetes mellitus has been paid more and more attention all over the world. In this paper，the development tendency of diabetes mellitus around the world and the research progress of low glycemic-index food were summarized. The food materials of low glycemic-index（starch，protein，fat and trace elements） and the effects of formulation of nutrients，the processing and the edible modes on the postprandial blood sugar level were also introduced in details. It would be useful for the further development of low glycemic-load food. Keywords： low glycemic-load，diabetes mellitus，combination of nutrients，processing technology，edible way

Abstract:In order to obtain the optimal condition of chymosin fermentation，we observed the influence of initial inducing OD600，methanol concentration and pH on the high density fermentation process in 3.6 L fermentor of P. pastoris GS115/pPIC9k-chy. By using glycerol feeding strategy with DO control in high-density fermentation and maintained the residual methanol concentration constantly with a methanol sensor. The result showed that the optimum condition were listed as follows：the OD600 was 150，the methanol concentration was 2.0% and the pH was 5.0，then the highest chymosin activity（17 80 SU/mL），the highest chymosin productivity（19.48 SU/（mL·h）） and the highest yield of products（2.42 SU/mL）were achieved，the total protein content and the ratio of milk-clotting activity to proteolytic activity（MCA/PA） were determined to be 0.64 mg/mL，63.43.

Abstract:Creatine amidinohydrolase（CRE） is one of the key enzymes for clinical determination of creatinine in serum and urine. In this study，the gene encoding for CRE was successfully amplified from Arthrobacter nicotianae 23710 and functionally overexpressed in Escherichia coli BL21（DE3）. The recombinant CRE was purified through a series of operation including ammonium sulfate precipitation，anion exchange chromatography and gel filtration chromatography. The specific activity of the purified enzyme reached 20.25 U/mg. The recombinant enzyme shows excellent resistance to the chelating agent EDTA，the surfactants（Tween20 and Triton X-100） and the common preservative NaN3.

Abstract:Soy sauce is a kind of Chinese traditional fermented condiment. The main mechanism of soy sauce production is the decomposition of raw protein into amino acids using protease formed by Aspergillus oryzae，and the late fermentation flavor，shower oil，sterilization and blending into. This paper studies the characteristics of the protease formed by Aspergillus oryzae in different temperature，pH and salinity conditions analysis，and by using BP neural network to optimize the protease hydrolyse conditions. The results show：the best effect condition of 42 ℃，pH 7.0，salinity is 0 °Bé. With the above research results as the foundation，carries on the scale of the experiment，the results showed that：the shop tests the optimal fermentation conditions for a temperature of 42 ℃，the whole salinity 18 °Bé，and salt water ratio is 1∶2，pouring 3 times/day.

Abstract:To explore the effect of σB in the growth of Listeria monocytogenes（LM），we compared the growth kinetics of LM-WaX12 and its dull sigB mutant at different pH values. We used splicing by overlap extension PCR（SOE-PCR） and homologous recombination to construct the sigmaB gene deleted mutant；The mutant was identified by the fragments that amplified by two sides primer of sigB. The growth curve of WaX12 and WaX12-sigB were fitted by modified Logistic model from pH 5 to pH 9，as well as both growth kinetic parameters were analyzed. The results showed the ?驻sigB mutant was constructed correctly. The maximum exponential growth rate（μmax），lag phase duration（λ） and maximum population density（MPD-OD） of WaX12-?驻sigB and WaX12 had all significant difference（p＜0.05） at pH 5，but had no different significant at neutral and alkali.

Abstract:L-asparaginase（L-ASNase） can catalyze the deamidation of L-asparagine（a precursor of toxic acrylamide） to L-aspartic acid and ammonia，and it is thus an important food enzyme. This study used Bacillus subtilis WB600 as the expression host，successfully realizing the secretory expression of L-ASNase. By using the atmospheric and room temperature plasmas（ARTP） mutation system，the titer of the recombinant L-ASNase was improved further. After 30 h fermentation，extracellular enzyme activity of recombinant B. subtilis WB600（pMA5- wapA -ansZ）reached 37.2 U/mL. This result suggested that L-ASNase can secrete to the medium mediated by a signal peptide of wapA. When treated with the ARTP at power input 120 W and gas flow rate 10 L/min for 40 s，a mutant of B. subtilis WB600（pMA5- wapA -ansZ）with a activity of 48.4 U/mL was obtained，the activity value was increased by 30%. These results suggested that ARTP mutation system can be used to improve the production of L-ASNase of recombinant strains. This study provided a good L-ASNase producing strain to fermentation production at an industrial scale.

Abstract:A codon-optimized mature peptide gene encoding a thermostable xylanase from Thermobifide fusca has been cloned into the expression plasmid pPIC9K and named as pPIC9K-xyl11M. The pPIC9K-xyl11M was linearized with SalⅠ and integrated into the genome of Pichia pastoris GS115 by electroporation. The recombinant P. pastoris GS115/xyl11M was screened by G418 and then was induced with methanol to express reXyl11M. The reXyl11M activity expressed by P. pastoris transformant reached 105.3 U/mL. The molecular weight of reXyl11M was estimated to be 34 000 by SDS-PAGE. The reXyl11M displayed the highest activity at 70 ℃ and pH 6.0. It was stable at a temperature range of 30~75 ℃，and at a pH range of 6.5~7.5. Co2+，Ba2+，Cu2+，Li+ had a strong enzyme activation effect，Fe3+ had obvious inhibitory effect，its activity was not significantly affected by other metal ions and EDTA. This revealed that reXyl11M was successfully expressed in P. pastoris and had good enzymatic characterizations.

Abstract:Malolactic enzyme（MLE） is a key enzyme inmalactic fermentation（MLF）during fruit-wine producing. In order to construct the secretory Escherichia coli（E.coli）expression system of MLE. The signal peptide gene（487 bp） and the MLE gene（1 623 bp） were combined through fusion-PCR，then the fused fragment was inserted into pET28a（+） and transformed into E.coil. 60 000 protein was obtained when the positive strain was cultured and induced with IPTG. 240.7 mg/L L-lactic acids could be detected in the fermentation supernatant with HPLC. All the results indicated the secretory E.coli expression system of MLE was successfully constructed. This will provide a technical platform for the research and application of MLE.

Abstract:Alkaline polygalacturonatelyase（PGL）is one of important industrial enzymes，which was widely used in food，textile and paper industries. In this study，we constructed and expressed six Self-assembling amphipathic peptides （SAPs） ，whihch were attached to the N terminus of the PGL，to improve the secretory expression of PGL in E.coli. Finally PGL-S1 stood out with enzyme activity improving from 129.65 U/mL to 535.47 U/mL，while the otherenzyme activity weredecreased. There was no obvious change in the extracellular yield of PGL by SDS-PAGE analysis. It indicated that the high enzyme activity was caused by the improving catalytic efficiency. The Grave Value showed that the hydrophobicity of SAP1 was different with the others，which was the main interaction for the accumulation of protein to enhance the interaction between protein and substrate. It was conferred that the improving surface hydrophobicity made a big effect on the catalytic efficiency. PGL-S1has huge potential use for industrial application.

Abstract:By using the lactic acid fermentation technology to prepare the soft-shell Palaemon carincauda Holthuis，the factors including temperature，time，inoculum and other factors were studied. The fermentation parameters were optimized by using response surface method. The best conditions of preparation soft-shell Palaemon carincauda Holthuis for lactic acid bacteria fermentation were obtained as follows：fermentation time 24 h，inoculum 108 cfu/mL，the amount of sugar added 20%，and temperature 25 ℃，under which the puncture peak of soft-shell Palaemon carincauda Holthuis reached to 169.4 g，and the pH of shrimp closed to 6.5 with well-preserved flavor.

Abstract:For investigation the application of protozoan Tetrahymena thermophila on food safety detection，two strains of protozoan T. thermophila Cu428.2 and B2086.2 were used as substrate to study the biotoxicity of food preservative sodium benzoate（SB）. Effect of SB on T. thermophila was assessed from changes in cell shape，movement style，population growth and superoxide dismutase（SOD） activity after adding SB to the culture of T. thermophila in Neff medium. Cell morphology and movement of T. thermophila were observed under inverted microscope at 6 h after adding 0%，0.01%，0.05%，0.1%，0.25%，0.5%SB to the culture. The results showed that in 0.01% and 0.05% SB，both strains of T. thermophila cells became shrunken and smaller，the speed of cell movement in the media was significantly slower than the control group. While in 0.1%~0.5% SB，cells morphology turned round from oval，holes appeared in the cell membrane，rupture and death cells were found. The higher the concentration of SB was，the greater damage of the cell membrane and the more the apoptotic cells appeared. Population growth curve and 24 h SOD activity of the two strains of T. thermophila cultured in Neff medium were determined in 0.000 1%，0.001%，0.005%，0.01% ，0.05% SB compared to the control group. The results suggested with the increase of SB concentration，population growth rate of T.thermophila decreased and 0.05% SB even killed the original seeded cells，however 0.000 1% SB promoted the population growth. While as the SB concentration increased ，the activity of T. thermophila SOD basically decreased，however，lower concentration of SB could enhanced the activity of SOD in a certain period of time.

Abstract:Pre-cultured Aspergillus niger was treated using ARTP for the development of high glucose oxidase strain. Improved O-dianisidine color-developing circle method was used for preliminary screening and the mutant strains were re-screened by liquid state fermentation in flasks. Mutant strain T-6's enzyme activity increased from 45.6 U/mL to 85.3 U/mL and was selected for its high glucose oxidase-producing ability. Glucose oxidase activity reached 125.6 U/mL which was 175.4% higher than that of the original strain when the critical fermentation conditions，calcium carbonate and Tween-80 were optimized. The genetic expression of glucose oxidase was showed to be stable when the strain was cultured for 6 generations.

Abstract:Abstract A strain designated as SH003 with high 2-phenylethanol tolerance and bioconversion properties was selected from 24 yeast strains with different sources. It could grow in the medium containing 4g/L 2-phenylethanol. Under the optimized conditions, the production and productivity of 2-phenylethanol respectively reached to 4.31g/L and 0.18g/L/h. The molar conversion rate of L-phenylalanine to 2-phecylethanol was 72.4%. Identified by the morphological and physiological characteristics and its 18S rDNA sequence, strain SH003 was closely related with Saccharomyces cerevisiae on phylogenesis.

Abstract:In order to investigate the effect of Enterococcus faecium and Bacillus subtilis mixed culture on their biomass，MRS medium，single factor experiment，Box-Behnken Design were adopted to optimize the mixed culture. Results showed that medium initial pH，liquid volume and nitrogen source had significant effects on the viable counts of the strains. The optimal culture conditions were as follows：initial medium pH 6.5，50 mL liquid medium in 500 mL flask with 180 rpm in shake，35 g/L of nitrogen source，the inoculation of Enterococcus faecium and Bacillus subtilis at a ratio of 1∶12. Under this conditions，the total viable counts reached to 6.99×1010 CFU/mL，with 4.58×1010 CFU/mL of Enterococcus faecium and 2.41×1010 CFU/mL of Bacillus subtilis，which was five times higher than that of before optimization.

Abstract:This paper took white shrimp granules as the text materials. Effects of different drying methods，hot air drying，microwave drying，microwave spouted drying，vacuum freeze-drying，on texture properties，rehydration ratio，color of dried white shrimps were researched. The results show that vacuum freeze drying to obtain the product quality is the best，but the drying time is hot air drying of 2.25 times，microwave drying and hot air drying and microwave drying of 9 times and 10.3 times；hot air drying can overcome the single microwave drying，and drying the sample quality is the most close to the vacuum freeze drying，so microwave spouted drying is a kind of development prospects.

Abstract:This paper discusses the antroquinonol purification process，the response surface method was used to extract more antroquinonol，and silica gel column chromatography to obtain high purity of antroquinonol .The experimental results show that the optimal conditions for the extraction were as follows：material ratio 1∶25（g/mL），the extraction temperature was 35 ℃，the extraction time was 85 min .The best conditions for the first silica gel column chromatography：sample volum 1∶40，diameter-length ratio 1∶30，elution flow rate 2.0 BV/h，the purity is 70.2%；The second optimum separation was achieved with eluant benzene∶acetone=7∶3（v/v），sample volume at 1∶40，diameter-length ratio 1∶10 and elution velocity at 1.0 BV/h，the purity is 91%.

Abstract:Combining the standard bacteria of Salmonella typhimurium and Salmonella paratyphi respectively with five other Enterobacter bacterium which are common in the usual determination to form into 10 groups of modal Bacteria-bearing Peeled Prawms，isolating and identifying them under the following present standards：GB 4789.4-2010，National food safety standard Food microbiological examination：Staphylococcus aureus. All the standard bacteria of Salmonella and the Enterobacter bacterium can be exactly detected from the 10 groups of test samples. The experiment shows that the characteristics of the colony on the BS would be more evident after culturing for 48h. And the longer the enriched culture cultures，the better the Salmonella grows. Salmonella paratyphi produces a little of H2S. Serratia marcescens inhibits the H2S-reaction of Salmonella paratyphi. Anti-O polyvalent serum of A-F of Salmonella can agglutinates Proteus mirabilis and Citrobacter spp. The determination techniques found in the test would be applied well in the determination of Salmonella for the aquatic products，animals and animal products.