Abstract:Polyunsaturated fatty acids（PUFAs），as functional oil components，have been widely used in food industry. The PUFAs produced from plants，mammals and deep-sea fish is inadequate for supplying the expanding market demands，and the content of vegetable and animal oils，types and proportion of unsaturated fatty acids in these oils also show limitations for further application. Microorganisms，especially algae and fungi，have been recognized as promising sources that can synthesize the entire range of PUFAs，and can be used as valuable alternative sources for PUFAs production on an industrial scale. This paper reviews the progress in the research of enhancement of polyunsaturated fatty acid production，based on the related research in culture condition，strain screening，gene engineering and metabolic regulation，providing theoretical and applicable references for relative domestic and international research.

Abstract:Aptamer as a single-stranded oligonucleotide can bind to target with high affinity and selectivity. Although many viable approaches for biosensing，diagnostics，and therapeutics have emerged，relatively few aptamers binding to small molecules exist. Small molecules are very important targets with diverse biological functions and clinical and co mMercial uses. Therefore，developing novel and effective molecular recognition probes for these compounds are greatly interesting. This paper will highlight various challenges during aptamer development relative to small molecule targets，as well as opportunities for their application in biosensing and food safety.

Abstract:In the present study，in order to improve the production of the recombinant Bacillus subtilis γ-glutamyltranspeptidase in E.coli BL21（DE3）/pET-20b（+）/ggt，the culture conditions and medium compositions were investigated and optimized in shake flasks. The optimal fermentation medium was as follows：10 g/L glycerol，21 g/L yeast extract，7 g/L peptone，and 130 mmol/L PO43+. In addition，the optimal culture conditions were firstly cultured at 37 ℃，200 r/min for 4 h，and then induced by 0.2 mmol/L IPTG at temperature 25 ℃ and 40~48 h. Under these conditions，the maximal enzyme activity reached 81.2 U/mL，which was 1.9 times as high as that not optimized.

Abstract:Cordycepin is the major effective component of Cordyceps militaris，which is getting more and more attention for its hygienic and medical effects. In this study，we obtained several strains with high cordycepin yield from Cordyceps militaris JN168 by using ion beam，nitrosoguanidine and ion beam-nitrosoguanidine mixed mutation. After further screened，the high yield compound mutation strain 2 was obtained. Furthermore，the liquid medium composition of this strain was optimized for the fermentation of cordycepin. The results showed that the components of optimized medium were as follows：glucose 40 g/L，yeast extract 25 g/L，MgSO4·7H2O 0.6 g/L，K2HPO4·3H2O 0.6 g/L，KH2PO4 0.6 g/L. With this optimized medium，the yield of cordycepin increased 5 times，reaching 1 045.65 mg/L.

Abstract:The ecological distribution of Aspergillus in the samples obtained at the key points of Jiangxiang liquor fermentation process in Maotai village and the effect of solid fermentation simulated by fresh bran medium on the growth of predominant strain Aspergillums hennebergii and the main stress conditions of glucoamylase and protease secretion were analyzed in this paper. One hundred and three strains were obtained and identified as A.niger，A.oryzae，A. terreus，A.tubingensis，A.flavus，A.hennebergii，A.fumigatus，A.niveus，A.candidus and Monascus，respectively. The suitable conditions for the growth and protease secretion of Aspergillums hennebergii were 50% water content of the fermentation substrate and the pH value of 3.5-4.0. The suitable growth temperature of Aspergillums hennebergii was 30~35 ℃，whereas the suitable enzyme secretion temperature was 32~35 ℃. High alcohol contents（>8%，v/w）inhibited the growth and enzyme secretion of Aspergillums hennebergii，while low alcohol contents（2%，v/w），lactose，and maltose promoted its growth and enzyme secretion. The salt of（NH3）4SO4 and NaNO2 and yeast could promote its growth，glucoamylase and protease secretion. Moreover，sorbose，sucrose and fructose could promote its glucoamylse secretion. The individual addition of sodium sulphate could promote its growth and enzyme production. Fresh bran，which was rich in carbon source，nitrogen source，inorganic salts and trace elements，could provide high-quality natural carrier for Aspergillums hennebergii growth and enzyme production. The application of Aspergillums hennebergii can satisfy the need of bilateral fermentation and flavor forming mechanism in traditional solid fermentation of liquor.

Abstract:Using frozen barley leaves as raw material，the effects of vacuum freeze-drying and spray drying on the solubility，bulk density，mean particle size，color difference and the key components of chlorophyll，flavonoids，SOD enzyme activity，and oxidation resistance of barley grass powder were studied in this paper. The results showed that spray-dried barley grass powder has better solubility，higher packing density，smaller mean particle size，and poor color in terms of physical characteristics. However，its content of the chlorophyll and flavonoids，and SOD enzyme activity of spray-dried powder were only 56.7%，68.1%，47.9% of vacuum freeze-dried powder，respectively. Moreover，the reducing power and DPPH· radical scavenging capacity of vacuum freeze-dried barley grass powder were greater than that of spray-dried powder. In summary，in order to get the barley grass powder with small size and good solubility，spray drying was a good choice，whereas vacuum freeze-drying was a suitable choice to obtain a high nutritional value and good color barley grass powder.

Abstract:In order to achieve the high cell density culture of Lactobacillus brevis H3，the effects of medium formula，culture conditions，neutralizers and feed batch methods on viable cell counts have been investigated. Suitable medium formulation are：25 g/L glucose，15 g/L yeast，1.53 g/L citric acid，18.58 g/L sodium citrate，20 mg/L VB6，0.58 g/L MgSO4·7H2O，0.25 g/L MnSO4·5H2O，1 g/L Tween-80. Optimal culture conditions are temperature 30 ℃，cultural fluid amount 40mL，pH 6.3～5.8，using 20% sodium citrate as neutralizer. Appropriate feeding culture method is 4% carbon and nitrogen sources （C/N ratio 5：3），which was added after 10 and 20 h，respectively. When fermented at the optimal culture conditions，the number of the bacteria cells was up to 1.27×1010 CFU/mL，which was 7.5 times as high as that when it was not optimized.

Abstract:In order to achieve the biodegradation of PVA1799 in the textile industrial waste water，an bacteria mixture with efficient degradation was screened and named as SH-TD. Three strains with PVA-degrading ability were isolated from the mixed culture SH-TD and nasmed as SH1（Aeromonas sp.），TD5（Pseudomonas sp.），and TD33（Acinetobacter sp.），respectively. The results from the single factor and Plackett-Burman experiments showed that Yeast-Extract，（NH4）2HPO4 and BaCl2 constituents have the significant influence on PVA degrading rate. Through ascent and Box-Behnken experiments，a response surface methodology（RSM） model was constructed. Data were analyzed by use of Design-Expert software. The optimal concentrations（g/L）of the variables were determined as （g/L）：Yeast-Extract 0.04，（NH4）2HPO4 48.42，BaCl2 0.06. Under the optimal conditions，the PVA degradation rate after 24 h increased from 30% to 80% .

Abstract:To express heterogeneous genes under the regulation of osmotic pressure in Candida glycerinogenes CGMCC NO. 6830，an integrative vector was constructed in this study. The constructed integrative vector harbored the backbone of plasmid pUC19，18S rDNA sequence of C. glycerinogenes CGMCC NO. 6830 as the integration site，phleomycin-resistant gene（ble） as a selectable marker，the osmolarity regulated promoter（PCggpd） and green fluorescent protein gene gfp as a reporter. The target gene was integrated into chromosomes of C. glycerinogenes CGMCC NO. 6830 and the expression level of gfp was regulated by osmotic pressure.

Abstract:This work studied the impact of hyperthermia acclimation and storage temperature on waterless storage of live Crucian Carps. Crucian Carps were hyperthermia acclimated at the speed of 5 ℃/h and 1 ℃/h，and then cultured for 24 hours at 4 ℃ and 0 ℃ in waterless ice storage rooms to observe variations activity of metabolic enzyme and antioxidant enzyme. The results showed that the temperature adaption set as 1 ℃/h increased the activity of Lactate Dehydrogenase（LDH）. The activity of isocitrate dehydrogenase（IDH） was maintained comparatively well under such condition，while brain succinate dehydrogenas （SDH） didn't change. Alaninetraensaminase （ALT） activity was maintained at 0 ℃storage，and Malondialdehyde（MDA） acitivity was accumulated at 0 ℃. No obvious change was discovered in liver，which was caused by accumulated Catalase（CAT） low temperature storage.

Abstract:AraE is an important unidirectional transport channel protein that could transport some compounds outside the cell. E. coli is a common host-bacterial to produce phenylpropanoids，and the growth was inihibited by phenylpropanoids. In order to investigate the transport ability of AraE on phenylpropanoids in E. coli，the gene overexpression and silencing routes was used in this study to regulate the expression of AraE. The level of overexpression and silencing was confirmed by qPCR and SDS-PAGE. The transport ability of phenylpropanoids was measured by detection of the recombinant bacterial growth kinetics parameter （μmax） in different typical phenylpropanoids （rutin，naringenin and resveratrol）. The result showed that AraE could only transport resveratrol in E. coli.

Abstract:This study aimed to investigate the effects of different enzyme accelerants on the production of aminopeptidase by recombinant Bacillus subtilis and develop an efficient extract process of aminopeptidase. The results indicated that the enzyme accelerants（KCl at 0.1 mol/L，Tween-80 at 2.4 μL/mL，L-glutamate at 0.6%，potassium tartrate at 0.7%） significantly increased the production of aminopeptidase. The cultural conditions were of 60 mL/250 mL of liquid，5% inoculation size，at 37 ℃，initial pH 7.5，shake flask rotation 220 r/min，and cultured time 36 h. Under these conditions，the aminopeptidase activity of recombinant Bacillus subtilis could reach 232.5 U/mL. Using the efficient extract process of aminopeptidase，the recovery and the purification fold of the L-leucine aminopeptidase could reach 67.23% and 8.29，respectively.

Abstract:To explore the effect of MAPW on physic-biochemiscal index of mixed tomato and green pepper，three different storage conditions，MAPW（silicone membrane areas：16 cm2；temperature ：（10±1） ℃），hypobaric storage（vacuum pressure：35~40 kPa；temperature：（10±1） ℃），and cold storage（temperature：（10±1） ℃ were used to store tomato and green pepper. The results showed that mixed tomato and green pepper stored in MAPW got better quality with a significant delay in changes of weight and color，the content of ascorbic acid，the decrease in soluble solids and titratable acidity，and the accumulation of malondialdehyde（MDA） in membrane lipid peroxidation products compared to the other two storage methods. It is concluded that MAPW can maintain the quality of the mixed pink-mature tomato and green bell pepper and thus prolong their shelf life.

Abstract:The effects of fermentation conditions on lactose oxidase activity producing by Raoultella terrigena Y20，which was screened by our lab，were studied by single factor experiment and response surface methodology. The results showed that the four independent variables as initial pH value，fermentation time，inoculation amount and fermention temperature had significant and sequence effects on the lactose oxidase activity. The optimal fermention conditions were as follows， inoculum amount of 2%，initial pH at 7.2，fermented 25 h，temperature at 28 ℃. Under the optimal conditions，the lactose oxidase activity was 74.96 U/g，which was almost consistent with predicted value of 78.87 U/g.

Abstract:Several carotenoids are synthesized by related enzymes in Erwinia uredovora，. Zeaxanthin diglucoside is synthesized by zeaxanthin glycosylase which was encoded by gene crtX. In this study，crtX of E. uredovora was amplified by PCR and cloned into expression vector. The recombinant plasmid was then transformed into E. coli BL21 （DE3） to construct the engineering bacterium. The recombinant zeaxanthin glycosylase was purified by Ni2+ chelating affinity chromatography and a DEAE-Sepharose FF ion-exchange chromatography. The purified protein catalyze the formation of zeaxanthin diglucoside in vitro.

Abstract:The preparation of nucleic acid reference materials of Salmonella Enteritidis（gDNA） was carried out in this study. A total of 200 samples were prepared，each of them contained 1 μg of Salmonella Enteritidis gDNA. Variance analysis results showed that F value was 0.835 7