Abstract:Analysis of amino acid in food and organisms is vital for people's health. Capillary electrophoresis characterized by low cost, less pollution, high efficiency and simple operation is widely used in analysis of amino acids. The developing and application of amino acids analysis by capillary electrophoresis combined with ultraviolet detector, mass spectrometry detector, fluorescence detector and electrochemical detector was reviewed. And capillary electrophoresis used in the chiral separation for amino acids was reviewed.

Abstract:A strain capable of producing urethanase was isolated from the mouse intestines and identified as Providencia sp. By analyzing the 16S rDNA sequence and conducting the physiology and chemistry experiment. The substrate specificity experiment showed that the enzyme can catalyze both urea and urethane with the optimal pH of 4.5 and optimal temperature of 35 ℃ but had no influence on the ester and amino acid. The fermentation condition was optimized based on single factor experiment. Under the optimal condition, the acid urethanase activity was increased from 0.32 U/mL to 0.63 U/mL and acid urease activity from 0.87 U/mL to 1.95 U/mL. The crude enzyme can effectively decompose the urea and EC in the Chinese rice wine and has little effect on the flavor components.

Abstract:A novel chitin-degrading aerobe, Chitinibacter sp. GC72, was isolated from a soil sample from Nanjing, and was proved to have high chitinolytic activity. The enzyme activity could reach to 2 835 U/g at 72 h of culture time, and one liter fermentation liquid contained 910 U chitinase. Its chitinase hydrolyzate, further investigated by TLC、UHPLC、Q-TOF-MS, was confirmed as GlcNAc. In the experiments of chitin hydrolyzation, the GlcNAc yield was 79.6% by using 3.44 U chitinase to hydrolyze chitin（200 mg） in 27 h.

Abstract:The existing synthetic methods of 3, 4, 6-tri-O-acetyl-1, 2-O-ethylidene -β-D- mannopyranose, 1, 2-O-ethylidene -β-D- mannopyranose, 4, 6-O- benzylidene-1, 2-O-ethylidene -β-D- mannopyranose were modified in this paper. And the same time they were synthesized by the microwave assisted method firstly, and the best results were got by orthogonal experiment based on single factor investigation. Their optimum technology of synthesis were 600 W, 3.5 h, 35 ℃, mass of sodium borohydride 2.5 g；600 W, 30 min, 30 ℃, mass of sodium methoxide 0.4 g；800 W, 1.5 h, 35 ℃, mass of p-methylbenzene sulfonic acid 0.2 g. Their optimized yield were 80.3%、98.3%、91.9%.

Abstract:The study were conducted to evaluate the commercial potential of Centropages mcmurrichi as aquatic food resources after a comprehensive analysis of its basic nutritional constituents, part of the minerals, Amino acid compositions and various fatty acid components of lipids via modern research methods of biochemistry . The determination results indicated that Centropages mcmurrichi（dry weight） contained a high level of crude protein（64.64%） as well as abundant minerals which consisted of Fe（339.28 mg/kg）, Zn（97.91 mg/kg） and Ca（3316.47mg/kg） respectively. In addition, it was not richer in the average percentage of fatty acid composition or the content of total sugar when compared with other marine copepods like Acartia clausi. The essential amino acids index（EAAI） was 80.51.Thirty-nine point fifty five percent of the total fifteen types of amino acids comprised of essential amino acids of which Leucine, Lysine and Valine were the most prevalent, and 60.45% were non-essential amino acids, Glutamic acid, Asparagine and Alanine. And Centropages mcmurrichi had special seafood flavor because of the five delicious amino acids in which glutaminic acid（7.65%） and aspartic acid（5.81%） made up a higher percentage. Fatty acid profiles of Centropages mcmurrichi showed that 40.65% saturated fatty acid, 16.05% monounsaturated and 42.09% polyunsaturated fatty acids occupied the total fatty acids, respectively. Among this detected eighteen diffident types of fatty acids, docosahexaenoic acid（DHA；22：6n-3） and eicosapentaenoic acid（EPA；20：5n-3） were the predominant fatty acids of Centropages mcmurrichi, which constituted 27.32% of total fatty acids. Therefore, Centropages mcmurrichi were esploitable as one of the high-quality marine aquatic resources.

Abstract:To overcome the poor water solubility or lack of tumor-targeting of Doxorubicin（DOX）, we designed and fabricate the redox-sensitive K5 polysaccharide micelles as drug carrier, methods：An amphiphilic K5PS-SS-DOCA（KSD） conjugate was designed and prepared by covalently coupling deoxycholic acid（DOCA） with K5 polysaccharide（K5PS） via disulfide bonds. The conjugate could self-assemble into micelles in aqueous solution and encaspulate model drug DOX. The morphology of the micelles was observed by TEM, and the size and ζ-potential were measured by DLS. Results：The micelles were of spherical shape. The average size and ζ-potential was 225 nm and -31 mV for the micelles, and 241 nm and -31 mV for the DOX-loaded micelles, showing favorable stability. The DOX-loaded micelles could behave redox-sensitive drug release behavior in the solution containing 10 mM GSH. In vitro cytotoxicity showed that the bare micelles were biocompatible and DOX-loaded KSD micelles were more cytotoxic against tumor cells than normal cells.

Abstract:Head Space Solid phase micro-extraction（HS/SPME） coupled with gas chromatography（GC） were used for the determination of volatile compounds in goat milk. HS/SPME experimental parameters, such as SPME fibre, extraction temperature, NaCl concentration, extraction time and desorption time were optimized using the number and area of peaks as index. The optimal extraction efficiency for goat milk was achieved when sample was headed at 70 ℃ for 90 min after the addition of 0.3 g/mL NaCl, extracted by a carboxen/polydimethylsiloxane（CAR/PDMS） fibre, and than the fibre was transferred to a GC injection port for thermal desorption at 250 ℃ for 5 min. As a result, 42 kinds of volatile components were detected that including acids（14 kinds）, esters（6 kinds）, hydrocarbons（5 kinds）, alcohols（4 kinds）, aldehydes（3 kind）, aromatic（5 kinds） and heterocyclic（5 kinds）.

Abstract:To develop a novel electrochemical immune biosensor based on antigen-antibody reaction with the immunoglobulin G immobilized the surface of the ITO. The side of ITO was immobilized the goat anti-human IgG after the amino-modified and glutaraldehyde modification. The side of SiO2 also had the same modification of amino and Glutaraldehyde, and then 2~5 nm gold nanoparticles adsorbing the surface of SiO2 by the use of electrostatic interaction. We immobilized the goat anti-human IgG after the reduction of H2O2 with bigger gold nanoparticles. When human IgG respectively combined with the ITO and nanomaterials, it would form the sandwich structure of a new detection of immunoglobulin G based on Au/SiO2 to amplify signal amplification. The prepared biosensor showed a excellent linearity relationship of Cyclic voltammograms current value and the concentration of immunoglobulin G 1~200 ng/mL, the equation of linear regression is y=-0.532 6x+409.256 7（R2=0.994 8）, with a detection limit of 0.6 ng/mL. The established method is low cost and ultrasensitive, and can be used to detect immunoglobulin G.

Abstract:Three Microorganism with SOD production were separated from the seafloor mud of Jianmen harbour in Taizhou, Zhejiang Province, China. Thus, further studying on one of the stronger SOD activity strain was undertaken, Its phenotype, genetic and phylogenetic analysis experiments suggested that the strain belongs to the family Bacillaceae, genus Bacillus and named as Bacillus cereus MBRH3. A sequential statistical methodology comprising of Contral Composite Design were applied to enhance the fermentative production of SOD, As a result, maximum chitinase activity of 203.38 U/mL was obtained in the optimized medium concentration（g/L） of 4.5 corn steep powder, 4.11 Sea weed paste, 2.10 glucose and 0.62 magnesium sulfate. Bacteria was deposited into China Center for Type CultureCollection（CCTCC NO：M2013581）.

Abstract:In order to clarify the effects of ultrahigh pressure processing（UHP） on the activities of cellulose, the CMC and FPA enzyme activities of cellulose in C. militaris juice were studied by pressure, holding time, temperature, ethanol concentration, times of pressure and pH. The result showed that the optimal pressure, holding time, temperature, ethanol concentration, times of pressure and pH of the enzyme were 400 MPa, 10 min, 35 ℃, 30 %, pH 4.0-7.0 and pressure for 1 times, respectively. Under the optimum conditions, the CMC enzyme activity were enhanced by 82.07%, 52.41%, 87.22 %, 54.48%, 67.52% and 66.67 %, and the FPA enzyme activity was enhanced by 97.37%, 61.4 %, 69.4 %, 61.4%, 1.31% and 33.91 %, respectively. The experiment illustrated that the ultra-high pressure treatment could enhance the activity of cellulose in C. militaris juice.

Abstract:Five kinds of process were applied to prepare pectin/chitosan（PEC/CS） composite membranes and the effect of membrane-forming conditions on the mechanical properties were explored, FT-IR, XRD and SEM were adopted to describe the structure of membrane. The results indicated that, the membrane prepared by dissolving the flocculent precipitate（formed by dissolving PEC/CS in the solution of acetic acid） with formic acid solution was smooth and transparent, and its tensile strength could reach 45.38 MPa, which was 1.42 times as that of gelatin membrane. The result of FT-IR analysis indicated that, a polyelectrolyte reaction happened between pectin and chitosan molecules through electrostatic and hydrogen bond interaction between -COOH and -NH2, and in this way, a composite material was formed. The result of XRD analysis showed that, the adding of pectin reduced the crystallinity of chitosan, a network structure was formed, and the mechanical property of the composite membrane was improved. The result of SEM analysis showed that, clear streaks could be observed in the surface of PEC/CS composite flocculent precipitate, and this indicated the poly-reaction occurred between pectin and chitosan molecules. Based on these experiment results.

Abstract:Four different Ventilation and stirring conditions were established , Taking CHO cells expressed Cetuximab as research object and adopting the serum-free mediumand suspension culture, the effect of different Ventilation conditions on cell density、cell viability and protein concentration were contrasted. The results show that compared with traditional Ventilation like micro bubble and no bubble, cell density and protein concentration of big bubble Ventilation was almost the same even better in some respects. The new aeration not only changed the microenvironment of the CHO cells, but also had a great advantage in the cleaning validation. This Study laid a foundation for the application of big bubble ventilation on Large-scale Mammalian Cell Culture, and also laid a foundation for the optimization of CHO culture technology in pilot scale.

Abstract:To investigate the bacteriostasis activity and tumour inhibitory effect of semi-synthetic derivate of isocorydine 8-amino-isocorydine (8-NH2-ICD). The bacteriostasis activities of alkaloid in vitro were tested with agar diffusion method and minimal inhibitory concentrations (MIC). Inhibitory activity on proliferation of tumour cells with treated by 8-NH2-ICD were measured by MTT assay. The results show there is a dramatic bacteriostasis activity on Salmonella typhi and Escherichia coli, and relatively mild effect on Enterobacter aerogenes. MGC803 cell and M059K cell proliferation effect were dramatically inhibited by 8-NH2-ICD in vitro. The anti-tumour activity was gradually enhanced with the alkaloid variety of dose. However, 8-NH2-ICD have been demonstrated with low toxity to normal fibroblasts MRC-5 till concentration up to 200 mol/L. To our knowledge, this is the first time that 8-NH2-ICD has anticancer and antibacterial activity is reported.

Abstract:Two kinds of dietary fibers were prepared from Cynomorium songaricum. Crushed stems of C. songaricum particles with diameters around 3 mm were defatted with ethanol. The defatted C. songaricum were divided into two portions. One portion was treated with cysteine and vitamin C solution and the other portion was treated with distilled water for 48 h at 60 ℃. Then the residues were collected by filtration and washed with distilled water. After being dried, physical properties of the dietary fibers were measured. The water retention capability and hydration capability of the dietary fibers pretreated with cysteine or without cysteine were 9.20, 5.83 gram and 7.5, 4.4 mL water per gram product, respectively. These data indicated that the dietary fiber pretreated with cysteine is better in quality than that pretreated without cysteine. High molecular weight condensed tannins could be degraded to small molecular derivatives by cysteine in acid solution. The small molecular derivatives could be washed out with water to obtain dietary fiber purer than the one prepared without cysteine.

Abstract:The aim of the research was to discuss the parameters for hydrochloric acid hydrolysis of wheat bran protein.This paper studies the hydrolysis process of wheat bran protein by acid, analyzed the impact of the concentration of hydrochloric acid, liquid ratio and hydrolysis time on the hydrolysis degree of hydrolysis of wheat protein extracted from wheat bran, and also analyzed amino acid composition of the protein hydrolyzate. The hydrolytic conditions for wheat bran protein were as follows：hydrolysis temperature of 100 ℃, hydrochloric acid density of 4 mol/L, solid-liquid ratio of l∶6, time of 18 h, the hydrolysis degree could reach 59.22%.The result of amino acid analysis of the hydrolyzate showed that the sum of the sweet and umami amino acids reached 53.77%. High levels content of sweet and umami amino acids of protein hydrolyzate could be obtained by hydrochloric acid hydrolysis, which provided the theoretical basis for the production of wheat bran protein hydrolysis.

Abstract:The Cd, Cr, Pb, As content of seaweed exceed the national standard. To make the heavy metal content in seaweed reduce to the national standard and provide basis for safe eating of seaweed, the response surface method was applied to optimize the conditions of the heavy metals removal from seaweed. The quadratic polynomial model of removal of heavy metals in seaweed was established and analyzed, which showed the best conditions of heavy metal removal from seaweed：the pH of removal liquid 2, time 4 h, the ratio of solvent to material 125 mL/g. Under these conditions, the content of Cd、Cr、Pb、As（total As）in seaweed were 0.75、0.63、0.81、14.95 mg/kg and the rate of removal were 61.14%、58.28%、70.97%、40.70%.

Abstract:The enzymatic characteristics of polyphenol oxidase（PPO） from Physalis pubes-cens L. was studied by spectrophotometer at 420 nm. The results showed that the PPO enzyme had the highest activity at pH 7.0 and 35 ℃ and the enzyme activity would been inactivated completely after exposure to 100 ℃ for 2 min. The kinetics of enzyme-catalyzed reaction of PPO was in accord with Michaelis-Menten equation, with Km and Vmax values of 0.025 mol/L and 125 U/min, respectively. In the range designated, it was increasing in inhibitory effects of ascorbic acid, citric acid, NaHSO3, L-Cysteine and EDTA against enzyme activity with the increasing concentration , and the order as follow：ascorbic acid﹥citric acid﹥L-cysteine﹥EDTA﹥NaHSO3.

Abstract:To determine the residual amounts of 16 Phthalic Acid in spirit, a liquid-liquid extraction combined with gas chromatography/mass spectrometry was established. The results showed that linearity of response over 16 phthalic acid esters was good from 0.5 to 8.0 mg/L with higher correlation coefficients（>0.997）.The limits of detection were from 0.02 to 0.05 mg/L, in addition, the relative standard deviations（RSDs） were in the range of 0.7%~2.8%. Spike recoveries ranged from 82% to 106% for all samples in same analysis condition. The conclusion indicated that this developed method is simple and quick response for the determination of 16 Phthalic Acid Esters in wine.