Abstract:Papaya proteinases was separated by Ion-exchange support; SP-Sepharose Fast Flow. The coagulability of chymopapain and papain to soy protein isolate was investigated by dynamic viscoelastic measurements and determination of hydrolyzing degree. The gel for

Abstract:以少孢根霉RT-3孢子接种在含高浓度的大豆异黄酮提取物制成的发酵基质中，发酵36h后大豆异黄酮被部分水解成相应的苷元．用从丹贝中分离的β-葡萄糖苷酶与标准品染料木素糖苷和大豆苷元的糖苷作用10min，染料木素糖苷和大豆苷元的糖苷被水解成相应的苷元．结果表明：丹贝异黄酮生物活性增强是发酵剂RT-3孢子分泌的β-葡萄糖苷酶将异黄酮由糖苷水解成苷元，而苷元比糖苷有更强的活性．

Abstract:Toluene has induced some diseases,screening of bacterial strains is necessary for toluene biodegradation. Five toluene biodegradation strains were isolated and screened from soil, and the time course of the bacteria growth were also determined. Strain ZD5

Abstract:研究了黄瓜切片在10℃，氙气压力0．3Mpa条件下的变化情况，黄瓜采后在空气常压，氙气0．1，0．2，0．3Mpa压力下放置12，24，48h后，过氧化物酶和多酚氧化酶活性的变化，并且作了氮气，氪气及氩气的对照．研究了黄瓜在0．3Mpa氙气处理24h后呼吸强度的变化，处理后的呼吸强度比对照组有明显的下降．

Abstract:To explore a method that can prevent haematopoietic cells from chemotherapeutic drugs during chemotherapy of tumor. MDR1 gene was transfered to cord blood nucleate cells (CBNC) mediated by adenovirus vector, for enhancing its resistance to chemotherapeuti

Abstract:通过采用真空预处理后进行气调包装技术，对生鲜牛肉冷藏过程中一系列品质的变化进行了研究，找到了延长牛肉货架期的较佳条件．主要考察了牛肉在不同的预处理终温、预处理压力及综合优化条件下保鲜效果的优劣．结果表明，选择预处理终温为4℃，预处理压力为1200Pa，联合气调包装充入体积分数40％的O2，25％的CO2气体时，保鲜效果较佳，可使牛肉货架期延长至14d以上．

Abstract:研究了银杏在SH、MS、B5、N6等培养基中的愈伤组织诱导培养，考察了在MS培养基中悬浮培养下溶解氧含量、激素浓度等对银杏黄酮苷生产的影响．结果表明：银杏在SH、MS、B5、N6等4种培养基中均可以诱导出愈伤组织，其中N6、MS培养基中诱导率最高，愈伤组织生长最好．悬浮培养结果表明，溶解氧、激素浓度对黄酮苷影响最大．

Abstract:Starch octenyl succinate anhydride (SSOS) with different degree of substitution was synthesized. The surface properties of different products were studied . The results showed that the emulsibility of the products increased with the increase of the substi

Abstract:人工合成的仿生鲶鱼抗菌肽DNA序列，经聚合酶链式反应(PCR)扩增后得到带有不同限制性酶切位点的序列XH1、XH2、XH3．将此基因克隆至载体pGEX-5X-3，成功构建了三串联的重组质粒．测序验证后转化大肠杆菌BL21(DE3)，37℃IPTG诱导，获得高效表达，SDS—PAGE扫描分析表明，融合蛋白可达细菌全蛋白总量的30％，融合蛋白以包含体的形式存在于大肠杆菌细胞中．

Abstract:真空预冷技术应用于双孢蘑菇的预处理，研究了双孢蘑菇在不同预冷终温过程中的温度、压力及品质的变化，并在随后的贮藏过程中，以呼吸强度、失重率和VC含量、细胞膜透性和感官品质为考察指标，从而确定最佳的预冷温度为5℃。经真空预冷处理的蘑菇的生理生化指标和感官品质均优于对照样．

Abstract:将来源于嗜热脂肪芽孢杆菌的启动子连同β-半乳糖苷酶bgaB基因经PCR扩增后,连接在T载体上,再取代枯草杆菌载体pZ01-bgaB的启动子,将其在枯草杆菌宿主WB600中表达.经摇瓶发酵20h,得到乳糖酶活力6.37U/mL,比活力3.814U/mg,SDS-PAGE电泳显示有明显重组蛋白质条带.证明了嗜热脂肪芽孢杆菌来源的启动子在枯草杆菌中是完全适用的.

Abstract:为了定向筛选α-糖苷酶抑制剂，建立了抑制α-淀粉酶、蔗糖酶和麦芽糖酶活试验为基础的筛选模型．采用此模型筛选从土壤和植物中分离的放线菌，其中菌株E-3519的发酵液对α-淀粉酶和蔗糖酶有强烈的抑制作用．菌株E-3519无气丝，基丝生长好，偶见孢囊，孢囊孢子游动迟缓；能水解淀粉，硝酸盐还原实验阳性，产生硫化氢，能使牛奶冻化；能利用大多数碳源；对枯草杆菌、大肠杆菌、金色葡萄球菌等无拮抗作用；可产生α-糖苷酶抑制剂阿卡波糖．E-3519主要生化特征及形态学特征与ATCC14539相似，命名为Actinoplanes utahensis var．。

Abstract:Mycelium biomass and polysaccharide accumulation in submerged fermentation by Boletus sp. ACCC 50328 are strongly influenced by many internal and external factors, namely medium constituents and fermentation conditions. the Plackett-Burman technique was a

Abstract:通过对一株淀粉液化芽孢杆菌进行紫外和^60Co逐级诱变，使该菌株产β-葡聚糖酶酶活从80U／mL提高到105U／mL；对突变株进行多次单菌落分离及传代，对其传代稳定性的研究表明：该菌株产酶性能稳定；在5L发酵罐中突变株的产酶水平比摇瓶要高，可达120U／mL，产酶周期比摇瓶缩短了15h．

Abstract:Effects of co-pigments on the color stability of bayberry (Myrica rubra) juice during storage were investigated. EDTA, Tween-80, L-proline, L-lysine, acetic acid and malonic acid had little effects on the color stability, while L-glutamic acid, purple swe

Abstract:研究了鹰嘴豆分离蛋白质质量、功能性质、物理化学性质及结构特征．氨基酸分析表明，鹰嘴豆蛋白含有所有必需氨基酸，与FAO／WHO模式相比，色氨酸是其第一限制性氨基酸，蛋白质的化学评分为71分．表面荧光探针ANS法测得的表面疏水性为94．3．DSC热分析显示该蛋白质含有两个热变性温度，分别为83．7℃和95．7℃．圆二色谱(CD)分析了鹰嘴豆分离蛋白的二级结构：α-螺旋36．2％，β-折叠28．4％，转角10．3％，无规卷曲25．2％．经凝胶过滤色谱Sephacryl S-200分离纯化得到7个不同相对分子质量蛋白质组分，其中两个主要组分的相对分子质量为170000及110000．

Abstract:对重组大肠杆菌产β-胡萝卜素发酵条件进行了研究．在M9培养基中添加Span-200．7g／L有利于细胞生长和β-胡萝卜素表达，初始pH值6．0、接种体积分数5％、发酵温度28℃、抗生素氨苄青霉素质量浓度80μg／mL和氯霉素质量浓度40μg／mL为最佳发酵条件，在7L发酵罐进行发酵动力学试验表明，重组菌最适发酵周期为22h，细胞干重最高可达1．55g／L，β-胡萝卜素表达量可达0．75μg／mL。

Abstract:不同厚度薄膜包装对水蜜桃品质变化的影响试验表明,气调包装显著保持果实可滴定酸水平,抑制总糖含量的降低.贮藏温度是影响水蜜桃MAP贮藏效果的重要因素之一.在(3±1)℃经40d贮藏,0.015mmLDPE的MAP失重率显著降低,小于3%,而对照为9.77%.低渗透薄膜(0.03mmLDPE和O.05mmLDPE)MAP果实的生理失调严重,果肉L*值增加.使用厚度为0.015mmLDPE的包装膜在(3±1)℃经40d贮藏,可保持包装内体积分数:O2为3%,CO2为5%.

Abstract:Applications of macerating enzyme in processing of vegetables and fruits were studied. Macerating enzyme was produced by Aspergillus niger. The condition of using macerating enzyme in processing of apple and cushaw was exten sively studied. The result sho

Abstract:对一种从灵芝发酵液中提取得到的蛋白酶A抑制剂GLPAI(Ganoderma Lucidum proteinase A inhibitor)的动力学性质进行了研究，分别以胃蛋白酶、胰蛋白酶和蛋白酶A为底物考察了GLPAI的动力学性质，实验结果：GLPAI对上述3种蛋白酶的抑制类型均属于混合型抑制模式，对胃蛋白酶的Ki-4．64(μmol／L)；对胰蛋白酶的Ki=33．5(μmol／L)；对蛋白酶A的Ki=2．7(μmol／L)．

Abstract:The fragrant compounds in cookie with chrysanthemum were isolated by solid phase microextraction and analyzed with GC/MS. 67 compounds were determined in the GC profile of chrysanthemum, occupying 77. 52% of the total fragrant components; 79 compounds wer

Abstract:The process of porous starch formation was observed by SEM, and the change of amylyse content and endothermic enthalpy during hydrolyzation occurred in sequence. This fact described the process of porous starch formation.

Abstract:通过单因素实验对影响碱提灵芝茵丝体多糖的各种因素进行了研究，确定提取条件为：提取温度65℃，提取时间4h，碱料(体积：质量)比4：1，碱液浓度0．5mol／L．在此条件下提取并对所得水溶性多糖的抗氧化作用进行了初步研究，表明其对羟自由基有较好的清除能力，在一定范围内清除率与糖的质量浓度呈正相关．

Abstract:为了测定绿色木霉固态发酵纤维素酶的生物量，采用紫外分光检测固态发酵过程中麦角固醇的量．确定了其最佳提取工艺：在75℃恒温水浴中反应0．5h，用乙醚萃取，乙醇定容，紫外282nm检测．在此基础上对绿色木霉固态发酵的培养基进行了初步的优化，并得到了最佳的培养基组合：碳源为蔗糖，无机氮源为(NH4)2SO4，水料质量比为2．2，pH为5．5．用该培养基，CMC酶活和滤纸酶活分别比优化前提高了21．0％和44．1％．

Abstract:Some Hansenula, Candida, Tor ulop sis and Pichia species of methylotrophic yeast have the growth capacity with methanol as sole carbon source. The heterogolous gene expression systems are characterized with the economics, safety. authenticity and posttran