2005, 24(2).
Abstract:Lactoferricin B (Lfcin-B) is the main antimicrobial peptide generated by pepsin digestion of bovine lactoferrin(BLf). Lfcin-B also shows a significant antibacterial activity than that of lactoferrin. In this paper, the antibacterial activity of Lfcin-B wa
2005, 24(2).
Abstract:A series of modern analysis methods, such as GC, HPLC ,LC/MS and NMR were used to analyze the composition and main structural features of P-WSPI1-S ,a purified fraction of wheat bran crude WSP. The molecular weight of P-WSPI1-S was 831,030, which consist
2005, 24(2).
Abstract:The effect of 6-BA on modified atmosphere packaging green asparagus was investigated in this paper. The results showed that, comparing with the CK, the treatments of 6-BA can decrease the respiration rate of asparagus and the climacteric. At the same time
2005, 24(2).
Abstract:对4个鸡腿蘑菌株进行了发酵筛选,选出生物量较高的菌株农林鸡腿蘑(NL),并通过单因素试验,确定玉米粉、蔗糖为碳源,麸皮为氮源;在此基础上,以筛选的碳源、氮源和无机盐KH2PO4和MgSO4·7H2O为考察因素,以生物量为主要指标利用正交试验优化培养基配方比例,确定鸡腿蘑摇瓶发酵培养基最佳配方为:玉米粉 4 g/dL,蔗糖 2 g/dL,麸皮 4 g/dL,KH2 PO4 0.1g/dL,MgSO4·7H2O 0.1 g/dL.
LU Fei , DOU Jun , LIU Ping , SUN Jun-she~
2005, 24(2).
Abstract:This paper investigated the protease of Actinomucor elegans AS3.227 with the help of ultrasonic,electrophoresis and high pressure liquid chromatography technology. The result showed that the mucor could produce both intracellular and extra-cellular prote
2005, 24(2).
Abstract:The effects of extrusion and tradition process on the edible quality of instant rice were studied in this paper. The extruded sample was faint white and opaque. The degree of gelatinization of this instant rice, the bulk density and rehydration rate were
2005, 24(2).
Abstract:Starch octenyl succinate anhydride (SSOS) with different degree of substitution was synthesized. The surface properties of different products were studied . The results showed samples with higher degree of substitution exhibited good surface activity char
FAN Liu-ping~ , ZHANG Min~ , HAN Juan~ , TAO Qian~ , XIAO Gong-nian~
2005, 24(2).
Abstract:Effects of two pretreatment, combined with vacuum frying on the moisture content, fat content and color value of fried green soy bean were studied. The results showed that pretreatment of hot air drying could reduce the fat content of green soybean, while
LIAO Jin-song~ , GUO Yong~ , ZHUANG Gui~
2005, 24(2).
Abstract:Bacteria with the ability to degrade Polyvinyl-Alcohol(PVA), were isolated from natural specimens. Two single resistant mutants, S7(Str~(s)Kan~(r)) and K15(Str~(r)Kan~(s)) were obtained by UV-irradiation. The S7 strain and the K15 strain could remove 52%
2005, 24(2).
Abstract:研究了中药黄芪对灵芝发酵生产灵芝多糖的影响，确定了黄芪添加的可行性及最适添加量，并对发酵产生的粗多糖进行分析，通过Sephadex G-75凝胶对粗多糖进行初步分离和研究．结果表明：黄芪有利于灵芝在发酵中多糖的产生，其多糖的组分发生变化，有可能产生新的物质。
2005, 24(2).
Abstract:为构建生物转化法生产茶氨酸的基因工程菌，作者分析了影响大肠杆菌高效表达γ-谷氨酰基转肽酶的几个主要因素．将大肠杆菌JM109的ggt基因接入两种不同的质粒中后，转化大肠杆菌JM109，并在一定的条件下进行表达．通过对比E．coli JM109和转化子的γ-谷氨酰基转肽酶表达活性的不同，探讨了ggt基因的拷贝数、启动子的强弱等几个因素对γ-谷氨酰基转肽酶高效表达的影响．实验表明，将含有自身信号肽和启动子的E．coli JM109的ggt基因接入高拷贝的质粒pUC18中，仅提高ggt基因的拷贝数不能增加γ-谷氨酰基转肽酶的表达量．将含有自身信号肽但不含有自身启动子的E．coli JM109的ggt基因接入具有tac启动子的表达载体pEtac中，发现在强启动子tac的控制下，γ-谷氨酰基转肽酶的表达量提高至出发菌株的2．3倍．将构建的工程菌用于生物转化法生成茶氨酸，底物转化率相应地提高至出发菌株的1．7倍．
2005, 24(2).
Abstract:在含50mL／L的甲醇、5g／L的甘氨酸的基本培养基平板上，从土壤中分离出130多株细菌，测定了它们利用甘氨酸生产L-丝氨酸的能力，获得了一株产L-丝氨酸较好的菌株A3，L-丝氨酸产量最高为1．4g／L，分类学鉴定为微球菌科(Micrococcaceae)，对菌株A3利用甘氨酸发酵生产L-丝氨酸的发酵条件进行了初步研究，包括甘氨酸和甲醇添加量，不同碳源、氮源，初始pH值以及发酵时间对产酸的影响．结果表明，菌株A3在不添加甲醇、只以甘氨酸为惟一碳源的发酵培养基中也能产L-丝氨酸，培养基中较高质量浓度的甘氨酸存在对L-丝氨酸生产是必需的．添加葡萄糖、甘油等外源碳源对菌体生长有利但不利于L-丝氨酸的生产。
ZHU Shao-juan , SHI Yong-hui~ , LE Guo-wei
2005, 24(2).
Abstract:研究了超声场下胰蛋白酶水解酪蛋白的酶解反应条件与动力学.在超声波频率 20 kHz、作用与间歇时间比为1∶1时,测定了不同功率下胰蛋白酶活性及该酶的最适温度、最适 pH和米氏常数Km 的影响.结果表明,180,225 W功率下,超声10 min的酪蛋白水解率显著高于常规对照组(P<0.05); 225 W超声功率下,在5 ℃,10 ℃,20 ℃下水解率分别比对照组提高 4.4 倍、2.47倍和2.33倍(P<0.01);超声场下胰蛋白酶 Km 值减小,Vm 增大.将胰蛋白酶、底物分别进行超声处理,紫外和荧光光谱分析观察到,超声处理后,酶溶液荧光发射光波长从334 nm移至332 nm,荧光强度提高,表明一定的超声波可能使酶及底物分子构象发生变化,提高胰蛋白酶水解活性.
LI Shu-yuan , ZHANG min , WANG Ya-nan
2005, 24(2).
Abstract:冷冻甘薯片在贮藏过程中发生的褐变与多酚氧化酶(PPO)的活性呈正相关．实验研究了甘薯片中多酚氧化酶的性质，并采用各种护色剂抑制甘薯片在冷冻加工过程中产生的褐变．结果表明，甘薯中多酚氧化酶在70℃下很不稳定，随着恒温时间的延长，活性迅速降低；甘薯中多酚氧化酶的最适pH值分别为5．0和7．5；甘薯片的最佳护色方法为热处理结合护色剂，L-半胱氨酸是较理想的护色剂，它不仅能够很好地抑制甘薯片在冷冻过程中发生的褐变，而且对甘薯的营养成分如VC具有保护作用。
2005, 24(2).
Abstract:Bacillus amyloliquefaciens is one of the few thermophilic bacteria. The phytase found in B. amyloliquefaciens has shown the extremely thermostablity and considerable potential in large-scale production. The gene of phytase BPhyC from B. amyloliquefaciens
BI Jin-feng~ , WEI Bao-dong~ , LI Chang-biao~
2005, 24(2).
Abstract:摘要：对产α-转移葡萄糖苷酶的黑曲霉U菌株固态发酵条件进行了优化．结果表明：麸皮和水的质量比为1：1较好，35℃培养48h时产酶量及酶活较高；U菌株生长的最适pH值为6．0；麸皮中的碳源和氮源可以满足该菌株产酶的需要，可以不外加碳源和氮源．
ZHAN Zhong-gang , ZHANG Min , AN Jian-shen
2005, 24(2).
Abstract:Inert gases form clathrate hydrates when dissolved in water, astrict the activity of water molecule, prolong the store of fruits and vegetables. The physiological and physical properties of asparagus spear were studied after treated by mixtures of argon a
ZHANG Chun-hua~ , ZHANG Min~ , SUN Jin-cai~ , ZHOU Le-qun~
2005, 24(2).
Abstract:Dehydrated cabbage pre-treated with glucose before drying easily become dim and then white in color during storage, due to glucose crystal transferring from inside to its surface. Fructose and high maltose syrup are used as the substitutes for part of gl
2005, 24(2).
Abstract:This paper shows the effect of different conditions of temperature and humidity on preservations of royal jelly and honey. The test indexes of royal jelly include the changes of water-soluble proteins, vitamin C, color difference and sensory indexes; the
SHAN Cheng-jun , CHEN Zheng-xing
2005, 24(2).
Abstract:研究了大米蛋白可食用膜的工艺条件，并测定了膜的抗拉强度、延伸率和透水率．实验表明，大米蛋白的质量浓度为5g／dL，甘油的添加量为3g／dL，谷氨酰胺转胺酶的添加量为质量浓度0．2g／dL，反应时间为90min，膜液在80℃处理40min后能得到性能比较好的膜。
2005, 24(2).
Abstract:Cellobiose and melibiose in the distillation of high-gravity ethanol broth from corn were assayed by HPLC method. The separation efficiency and recovery ratios of the process were calculated. The results showed that the analytic effect for detecting the
HAN Shao-qing , ZHAO Qin , PENG Qi-jun
2005, 24(2).
Abstract:The extraction of yeast extract by ultrafiltration and nanofiltration were studied with an attempt to develop a possible separation and purification process. The results indicated that 96% of proteins was blocked by the membrane of MWCO 5000. The operatio
CHEN Ya-ping , XIANG Ben-ping , HUANG Lin
2005, 24(2).
Abstract:A new slurry agent was synthesized by oxidization of the starch with hydrogen peroxide under a certain oxidization degree, and then reacting with a cross-link agent. The effects of hydrogen peroxide volume and reaction pH on oxidizing deree were also anal
SHU Jian-cheng~ , JIN Zheng-yu~
2005, 24(2).
Abstract:本试验研究了在经膨化处理的陈化早籼糙米日粮中添加外源α-淀粉酶和糖化酶对断奶仔猪小肠黏膜二糖酶、血液生化指标和生产性能的影响．试验为日粮不同加工处理(膨化、不膨化)和加外源酶(添加、不添加)两因子设计，共4个处理．结果表明，试验猪的生产性能总体趋势是非膨化组好于膨化组，非膨化加酶组的日增重比膨化加酶组在第14天和第28天分别高出23．66％和16．83％，料肉质量比降低了16．88％和7．36％；膨化和添加外源酶显著提高了断奶仔猪试验第14天十二指肠中麦芽糖酶的活性，然而膨化显著降低了断奶仔猪试验第28天空肠中乳糖酶、麦芽糖酶、异麦芽糖酶和蔗糖酶的活性，添加外源酶显著提高了断奶仔猪试验第28天空肠中乳糖酶和麦芽糖酶的活性．试验结果表明，对断奶仔猪高糊化度膨化陈化糙米是没有必要的．
SUN Su-ling~ , GU Xiao-hong~ , TANG Jian~ , YUAN Shen-shu~ , WANG Li-ping~ , LIU Yang-min~
2005, 24(2).
Abstract:Seal oil was derivated with heterocycliczation method and methyl esterization method respectively, and analyzed with GC/MS. The result showed that, Ge profill had 30 peaks, 30 compounds were indentified, and the double bonds in long-chain polyunsaturated
Copy Right：Editorial Board of Journal of Food Science and Biotechnology
Address：No. 1800, Lihu Avenue, Wuxi 214122, Jiangsu Province,China PostCode：214122
Phone：0510-85913526 E-mail：xbbjb@jiangnan.edu.cn
Supported by:Beijing E-Tiller Technology Development Co., Ltd.
