Abstract:In this study, synthetic wastewater was treated by a pilot-scale Anaerobic Sequencing Batch Reactor (ASBR)with the working volume of 100 L. The effects of feeding time, agitation and organic loading rates on the performance as well as anti-shock capabil

Abstract:Samples including lung, kidney, spleen, mesenteric lymph node, thymus, gonads, muscle and liver were obtained 3 weeks after oral administration of pcDNA3s plasmid in mice. Genomic DNA can be assayed for integrated plasmid by PCR after purification of high

Abstract:探讨了红曲中黄曲霉毒素B1的提取方法,发现在传统提取方法甲醇/水溶液(体积比1∶1)直接提取的基础上加氯仿进行液液萃取后,进行ELISA法测定其含量,可以消除假阳性,同时提高了检测灵敏度.在加标质量分数分别为2.5,5,10 μg/kg时,加标回收率达到101%～146.8%,方法的重复性较好,变异系数小于13%,样品最低检测限达2.5 μg/kg.

Abstract:High hydrostatic pressure (HHP) is an effective cool technique of inactivation. Screening design was used for estimating the sterilized effects of external factors in High hydrostatic pressure in this study, with respect to the variables such as pressure

Abstract:在HPLC分析唾液酸质量分数的基础上,选择盐酸作为大肠杆菌发酵液中聚唾液酸的水解用酸.在85℃水浴.盐酸浓度为0.1mol/L的条件下,水解2h,水解率平均可达95%以上.水解液中和过滤后,用离子交换色谱分离与冷冻干燥得到唾液酸产品.质谱及HPLC分析证实所得产品中主要成分是N 乙酰神经氨酸,其纯度达96.4%.

Abstract:研究了双突跃电位滴定法测定壳聚糖脱乙酰度的准确性以及壳聚糖溶液质量浓度、粘度、不同脱乙酰度等因素对滴定终点测定结果的影响.结果表明:壳聚糖样品质量浓度控制在2～6g/L,利用双突跃电位滴定法可以测定壳聚糖的脱乙酰度,不同样品脱乙酰度测定结果RSD(相对标准偏差)小于0.61%(n=3),该方法具有较高地准确性,可以用于壳聚糖生产和加工过程中的质量控制与检测.

Abstract:采用摇瓶培养法对黄伞菌丝深层发酵培养条件进行了研究.通过正交试验初步确定黄伞菌丝深层发酵适宜的培养基组成为:葡萄糖3g/dL,牛肉膏1.5g/dL,K2HPO40.5g/dL,MgSO40.1g/dL.该菌株最适培养条件为:培养温度25℃,起始pH值5.0,接种体积分数15%,发酵周期10d.在优化的试验条件下,进行摇瓶发酵,菌丝干重达11.16g/L.

Abstract:Starch octenyl succinate anhydride (SSOS) with different degree of substitution was synthesized. The rheological properties of different products were studied by rotary viscosimeter. The results showed the rheological properties of starch pastes changed a

Abstract:Soybean is widely attractive because of its biologic active substances such as fatty acid, amino acid and soybean isoflavones. In this study, the components and kinetics of the soybean classified extration from the industrial residues. A plenty of compoun

Abstract:对同步脱氮过程中影响N2O产生的条件进行了研究.结果表明,由于受反硝化反应影响,COD/NH+4-N比值为2,3时产生较多的N2O,分别为15 mg/L和25 mg/L;而比值为4,5时N2O生成量较少.同样,较高的溶氧质量浓度(3,4 mg/L)减小了颗粒污泥内部的反硝化区域,反应产生较多的N2O,控制DO质量浓度在1～2 mg/L,有利于减少N2O的排放.脱氮过程中添加NO-2-N和NO-3-N,反应产生大量的N2O,最多可以达到75 mg/L. 实验发现,NO-2-N较NO-3-N更易形成N2O.

Abstract:对管碟法测定Nisin效价的条件进行了研究,考察了几个参数的影响,得出了Nisin测定的最佳条件:90mm培养皿中培养基加量为15mL,Na2HPO4·12H2O质量浓度1g/dL,菌悬液浓度109CFU/mL,琼脂质量浓度1g/dL,培养基pH值7.0,牛津杯中样品加液量100μL.在此条件下,Nisin效价在5～100IU/mL,其对数值与抑菌圈直径有较好的线性关系.

Abstract:Extraction of COD from the supernatant broth was difficult, because the addition of additive W emulsified with COD. The addition of 2-propanol and(NH_(4))_(2)SO_(4 )to supernatant broth could effectively break microemulsion and remove additive W, the rec

Abstract:The experiments investigating the properties of antioxidant activity,ACE inhibitory activity, and molecular weight distribution of the soybean polypeptides with DH of 10%~24% producted by AS1.398 and Alcalase, were conducted.The results indicated that the

Abstract:利用超滤和平衡渗析两种技术脱除溶丝液中的CaCl2,结果表明,溶丝液经中空纤维超滤脱盐,其脱盐率达86.57%,回收率达74.20%,氮溶解指数(NSI)达3.40%;而经平衡渗析脱盐,其脱盐率达98.5%,回收率达83.13%,氮溶解指数(NSI)达97.85%.因此,平衡渗析是适合制备可溶性丝素蛋白技术.

Abstract:超声波破碎黑曲霉细胞提取游离果糖转移酶,然后选择D201大孔阴离子交换树脂为载体,通过先吸附后交联的方法固定果糖转移酶.优化的固定化条件:加酶量为每克湿树脂400U,吸附pH值为5.0～5.5,吸附温度为30℃,吸附时间为8h,交联剂戊二醛(终)质量浓度为0.01～0.05g/dL,交联时间为8h,交联温度为1～4℃.固定化酶活最高回收率为30.2%.

Abstract:利用固载的磷钨酸作催化剂,对中碳链脂肪酸和甘油的酯化反应进行了研究,考察了时间、催化剂用量、酸醇摩尔比和温度对酯化反应的影响,确定其最佳的反应条件为催化剂的用量为甘油质量的4%,反应体系的温度为初温140 ℃,终温160 ℃,残压10 kPa,酸醇摩尔比为3.1∶1.反应时间为1.5 h,最终酯化率可达89%.

Abstract:从婴儿粪便和药品中筛选到耐酸和胆汁盐的2株双歧杆菌菌种,分别命名为Bi.bifidumWN 04与Bi.longumGB 03.在pH值2.0条件下培养2h后再于胆汁盐质量浓度1.0g/dL的条件下培养2h.2株菌在pH值分别为2.0和3.0的50mmol/L的磷酸盐缓冲液中存活率较高;在胆汁盐质量浓度分别为0.25,0.5,1.0g/dL和2.0g/dL,pH值为6.8的50mmol/L磷酸盐缓冲液中存活率也较高,胆汁盐耐受性没有明显区别.在4℃保存于新鲜牛乳中,Bi.bifidumWN 04能够在8周内保持最高活菌数[lg(CFU/mL)>9],其它菌种活菌数[lg(CFU/mL)]<8.

Abstract:Four kinds of methods, thin-film, sonication, reverse-phase evaporation and freeze-thawing were used to prepare liposomes of ferrous sulfate. The operational conditions of preparing liposomes of ferrous sulfate were determined via single factor trial and

Abstract:研究了Zn2+在SK4.001发酵生产转谷氨酰胺酶中的作用. 在发酵培养基中加入不同质量浓度的Zn2+,检测菌体量、pH、残余甘油及转谷氨酰胺酶(TGase) 酶活的变化, 发现Zn2+在SK4.001发酵生产转谷氨酰胺酶中除满足菌体生长需要外,还具有提高转谷氨酰胺酶酶活的作用. 发酵培养基中含有3.15 μg/mL Zn2+时,菌体生长正常,菌体量较高;当Zn2+质量浓度增加到8.15 μg/mL时,菌体量改变不明显, 但转谷氨酰胺酶酶活迅速上升,达到4.723 U/mL,是Zn2+质量浓度为3.15 μg/mL时的2.06倍.

Abstract:脆壁克鲁维酵母(Kluyveromycesfragilis)LFS 8611合成的β D 半乳糖苷酶具有较高的催化半乳糖基转移反应活力.脆壁克鲁维酵母(K.fragilis)LFS 8611细胞生长和β D 半乳糖苷酶的合成同步.该菌株生长和产酶的最适碳源为半乳糖,乳糖次之;最适氮源为蛋白胨F403;最适培养条件为:发酵培养基的初始pH值为7.0,摇床的转速为200r/min.培养基中碳源和氮源质量浓度对菌体生物量和β D 半乳糖苷酶活力有重要影响,以12mg/mL乳糖为碳源,16mg/mL蛋白胨(F403)为氮源,在最适培养条件下培养32h后,菌体生物量和β D 半乳糖苷酶活力分别为7.56g/L和18.83U/mL.

Abstract:The formation and stability of colloidal gold Nano-particles in different diameters by means of sodium citrate reduction was studied in this paper. Reaction kinetics process of the solution behavior of colloidal gold formation was performed at different t

Abstract:As a food additive, blended glucoamylase activity is the major quality control criteria. With a suitable activity test method, the real glucoamylase activity in the blending product can be detected from synergistic effect be eliminated. In this article,

Abstract:构建重组菌(JM109/pTrc 99A xylIV)作为极耐热木聚糖酶的生产菌株,研究了IPTG诱导时间、IPTG浓度、重组菌生长的不同阶段加IPTG和温度对重组菌生产极耐热木聚糖酶的影响以及它的酶学性质.结果表明,IPTG诱导8h后极耐热木聚糖酶的表达量基本维持不变,0.5～5mmol/L浓度的IPTG诱导重组菌表达极耐热木聚糖酶的效果基本相同;当重组菌生长到OD600为1.2左右时加IPTG诱导最好,诱导温度对极耐热木聚糖酶表达水平的影响不大.重组耐热木聚糖酶的最适反应pH值为5.4～5.8,重组极耐热木聚糖酶的最适反应温度大于100℃,重组极耐热木聚糖酶在pH值4.2～7.5都比较稳定,重组极耐热木聚糖酶的温度稳定性好,在90℃下保温2h后残存酶活还有90%.

Abstract:研究了革兰氏阴性细菌大肠杆菌JM109和革兰氏阳性细菌地衣芽孢杆菌0204在电转化过程中,高渗溶液如山梨醇、甘露醇、甘油、蔗糖和葡萄糖对其电转化率的影响.结果表明,高渗溶液能够有效保护细胞,提高转化率.在复苏培养基中,0.7mol/L甘露醇对地衣芽孢杆菌0204的保护效果最好,0.1mol/L蔗糖对大肠杆菌JM109的保护效果最好.由此得到质粒DNA对地衣芽孢杆菌0204的最高电转化率为9.8×102转化子/μg,质粒DNA对大肠杆菌JM109的最高电转化率为5.9×108转化子/μg.

Abstract:采用高效液相色谱法测定了山楂、决明子颗粒剂中大黄酚的含量.以VP ODS(4.6mm×150mm)为色谱柱,流动相甲醇∶质量分数0.1%磷酸溶液(体积比)为90∶10,检测波长254nm,柱温为室温;大黄酚平均保留时间为7.27min,平均加样回收率为99.33%,RSD为1.87%(n=4),线形范围2.9～29.0μg/mL(r=0.9999,n=6),样品大黄酚的质量分数为0.0557%.该方法准确,灵敏度高,可作为该制剂的质量控制标准.

Abstract:This paper discussed the function, status, and trend of rice production in China. Limited benefit in rice production is mainly because of the low utilization of rice resources. Intensive utilization technology of rice has become one of bottleneck factors