Abstract:A rapid and simple method was developed for determination of aroma components of apple wine by head space solide phase microextraction(HS SPME) coupled to capillary gas chromatography mass spectrometry (GC MS). Four parameters, including fiber coatin

Abstract:苹果中的多酚氧化酶是引起苹果及苹果酒褐变的重要原因之一 ,用乳化剂及酚结合剂从苹果中提取多酚氧化酶 (PPO) ,研究其酶学性质 :不同底物的Km、Vm,反应的最适温度、pH及两种底物同时存在时的酶学效应 .结果表明 :4 甲基儿茶酚为最适底物 ,其最适温度 30℃ ,最适 pH4 .5 ,不同浓度咖啡酸对酶的影响不同 ,儿茶素对不同浓度绿原酸的酶促反应影响也不同 .研究几种效应物对酶活力的影响表明 :偏重亚硫酸钠、半胱氨酸、抗坏血酸为强烈抑制剂 ,羧酸类对酶具有抑制效应 ,肉桂酸比同一结构形式的苯甲酸抑制效果强

Abstract:In the induction phase of fed batch cultivation of recombinant Pichia pastoris with a Mut s phenotype, methanol concentration is significant for angiostatin expression. Due to the extremely slow methanol utilization by this Mut s strain, methanol w

Abstract:Aureobasidium pullulans releases a melanin like black pigment during the cultivation and the pigment was tightly adhered to the pullulan. The feasibility of 60 Co mutagenesis for pigment free pullulan production was studied on Aureobasidium

Abstract:Thirty two strains of enteric bacteria were isolated using xylose as sole carbon source. Identification was carried out and the results indicated that most of these strains were Klebsiella pneumoniae and then Escherichia coli . Appropriate plasmids

Abstract:从产果糖转移酶的 11株菌株中筛选出一株黑曲霉VVTP84菌 .该菌株在含蔗糖的培养基中 ,最佳摇瓶发酵时间为 30h ,pH值为 7.0 ;当蔗糖质量浓度在 2 5 0g/L以内时 ,产酶与蔗糖质量浓度呈正相关 ;MgSO4 ·7H2 O和KH2 PO4 的添加量分别以控制在 1.5 g/L和 1.0 g/L为宜

Abstract:探讨了乙醇体积分数、卡拉胶质量分数以及卡拉胶与糖、盐共存时对牛奶酒体系稳定性的影响 .结果表明 ,随着乙醇体积分数的增加 ,牛奶酒体系粘度呈线性增加 ,当乙醇体积分数超过30 %时其稳定性被破坏 .卡拉胶的加入会对体系的稳定性产生一定的改善作用 ,而柠檬酸三钠的添加 ,能使这种改善作用明显提高 .添加质量分数为 10 %的蔗糖对稳定性不能起到改善作用 ,当柠檬酸三钠和蔗糖共同存在时 ,对体系的稳定性影响以蔗糖为主 .同时 ,在柠檬酸三钠存在下 ,将添加卡拉胶的牛奶酒在 4 0℃条件下进行加速贮藏试验 ,测定了这一过程中pH值、粘度和色度的变化

Abstract:计算机控制的微型生物传感器分析系统可以实现生物反应过程中多参数的在线检测 .系统包含在线微量取样、级联稀释、微型生物传感器、生化工程数据处理和分析软件与计算机 .系统实现的指标为取样 10～ 10 0 0 μL/min ,稀释倍数 10 10 .5 4,相对误差小于 2 .5 % ;在线测量酵母发酵的葡萄糖质量浓度 ,最大误差率为 3.88% .

Abstract:An improved mechanical(semi continuous) model including 25 process variables and a series of cost variables was set up for repeated fed batch penicillin fermentation process in an industrial scale bioreactor. The model is based on research of fermentat

Abstract:Saccharomyces B5 and Candida 104 which have activity of 3 chloropropiophenone reduction to R 3 chloro 1 phenylpropanol were isolated. The effect of co substrate, reduction time, pH, temperature, substrate concentration and cell dry weight on

Abstract:Based on the reaction among antibody antigen enzyme antibody, using purified Bt1 insecticide crystal protein as standard protein and immunity antigen, a quantitative indirect enzyme linked immunosorbent assay (ELISA) method was established to detect Bt

Abstract:利用柱层析法从大豆胚芽中分离到 8个异黄酮化合物 ,分别是黄豆素糖苷、大豆苷元糖苷、染料木素糖苷、丙二酰黄豆素糖苷、黄豆素苷元、大豆苷元、染料木素、染料木素葡萄糖苷 - 6″ -阿拉伯糖 ,其中化合物染料木素葡萄糖苷 - 6″ -阿拉伯糖在大豆中为新发现

Abstract:从肺炎患羊的肺叶中取得微生物 ,经菌落分离、镜检、生化反应鉴定 ,分离出兽疫链球菌 .通过正交实验确定摇瓶培养最优培养基为 :葡萄糖 6 0 g/L ,蛋白胨 10g/L ,酵母抽提物 10 g/L ,K2 HPO4 2 .0 g/L ,(NH4 ) 2 SO4 5 .0 g/L ,MgSO4 · 7H2 O 0 .5 g/L .筛选出Streptococcuszooepi demicucNUF 0 35菌株 ,该菌产生的粗品透明质酸的相对分子质量为 2 .11× 10 6 ,产量达 1.88g/L.

Abstract:以蔗糖、果胶、羧甲基纤维素或木聚糖为碳源培养草菇 (Volvariellavolvacea) ,对所产生的半纤维素酶进行初步研究发现 ,半纤维素酶系主要是由木聚糖诱导 .半纤维素酶中的木聚糖酶分布在胞外 ,木糖糖苷酶分布在胞内 ,阿拉伯糖苷酶胞内胞外都有分布 .木聚糖酶的最适反应温度为 6 0℃ ,最适反应 pH为 5 .8,在pH 5 .4～ 7.0时比较稳定 ,保温 1h酶活的半衰温度是 5 5℃ .木糖糖苷酶的最适反应温度为 5 5℃ ,最适反应 pH为 6 .6 ,在 pH 6 .6～ 7.4时比较稳定 ,保温 1h酶活的半衰温度是 5 2℃ .阿拉伯糖苷酶的最适反应温度为 6 0℃ ,最适反应pH为 5 .0 ,在 pH 4 .6～ 6 .2时比较稳定 ,保温 1h酶活的半衰温度为 6 0℃ .

Abstract:Several factors influencing CoQ 10 production with Rhizobium radiobacter WSH2601 were investigated. The results showed that yeast extract and corn steep liquor are suitable nitrogen sources, and the carbon sources such as glucose and sucrose are

Abstract:为了更好地保留牡蛎制品中的有效成分 ,将牡蛎肉直接打浆均质后 ,加入粘结剂等制成片剂 ;同时 ,为了更好地对牡蛎进行综合利用 ,将牡蛎壳粉碎后加辅料 ,制成钙片 ,可以作为钙补充剂 .实验结果证明 ,牡蛎肉压片的最佳工艺为 :选择体积分数为 75 %的乙醇溶液作为润湿剂 ,烘干温度 6 0℃ ,烘干时间 4 0min ,水分质量分数控制在 5 %～ 6 % ,加入质量分数 1%的硬脂酸镁作为润滑剂 ;牡蛎壳压片的最佳工艺为 :控制水分质量分数在 6 %左右 ,选用m ( β 环糊精 )∶m (麦芽糊精 )∶m(可溶性淀粉 ) =1∶2∶4的混合辅料 .

Abstract:采用柱前衍生反相高效液相色谱法 (RP HPLC)测定碱性氨基酸含量 ,衍生化试剂为 2 ,4 二硝基氯苯 (CDNB) ,流动相为 0 .2mol/L的醋酸 醋酸盐缓冲液 ( pH 5 .2 0 )和甲醇的混合溶液 ,其体积配比为 75∶2 5 ,不需梯度洗脱 ;采用C18柱 ,在波长 36 0nm条件下进行紫外检测 ,碱性氨基酸质量浓度与峰面积呈良好的线性关系 ,相关系数均在 0 .98以上 ,分离效果较好 .

Abstract:以Ce4 +为引发剂 ,研究了玉米淀粉 丙烯酰胺接枝共聚物形成的条件 .结果表明 ,引发剂用量、单体用量、反应温度及反应时间对合成产物的接枝率和单体聚合率有很大的影响 ,当淀粉用量为 5 .0 g ,引发剂用量为 1.0mL ,温度为 6 0℃ ,单体用量为 8.0 g时 ,单体聚合率最大 .另外 ,还研究了淀粉用量 ,Ce4 +-S2 O2 +8复合引发剂对玉米淀粉与丙烯酰胺接枝共聚的影响

Abstract:The properties of static and dynamic adsorption and desorption of macroporous resin for soybean isoflavone were investigated.The D4020 resin was selected to purify soybean isoflavones in solutions. The purity of the product is over 40%,and can satisfy

Abstract:以罗非鱼的鱼内脏为研究对象 ,采用钾盐蒸煮法从罗非鱼内脏中提取鱼油的工艺条件和纯化不饱和脂肪酸 (PUFA)的方法 ,对精制鱼油的氧化性及人工合成抗氧化剂 (TBHQ)、VE、茶多酚 3种抗氧化剂对鱼油的抗氧化性能进行了研究 .通过正交试验及对比试验 ,结果表明 ,从罗非鱼内脏中制取鱼油的最佳工艺条件 :水解温度 70～ 80℃ ,水解时间 4 0min ,KNO3用量 6 g/dL ,盐析时间 10min ;采用低温 钾盐乙醇法纯化鱼油PUFA的效果比尿素包埋法好 ;TBHQ、VE、茶多酚 3种抗氧化剂对鱼油抗氧化作用以茶多酚效果最好

Abstract:The method of steam distillation was carried out to extract the volatile oil from Rhizoma curcumae . The extraction process was optimized by orthogonal design. The best condition was: using Rhizoma curcumae rough powder as raw material, adding 8 fo

Abstract:This article described several blotting methods of SDS polycrylamide gel electrophoresis to analysis low molecular weight polypeptides. The result showed that when the coomassie brilliant G 250 was used to stain the gel, the band of the peptide with

Abstract:About 300 strains of actinomycetes were screened for their capacity to convert lovastatin by thin layer chromatography and HPLC. Strain ST48 was found to transform lovastatin effectively. The effects of the age of the culture, dry weight of biomass, and

Abstract:The effects of ultrasonic radiation on the production of monascus pigment were studied. Ultrasonic wave with low intensity at 20 kHz, 200 W/cm 2 was employed to study the effects of irradiation at different length of time on the growth ofm Mona

Abstract:In this article, the procedure of extracting egg yolk oil from egg yolk powder by enzymes was studied. Through orthogonal experiments, the best technical conditions of enzymatic extraction of egg yolk oil were determined as followings:the concentration o

Abstract:通过 4组实验对照 ,在生产鸭肉香肠时加入不同含量的 β 环糊精 ,按照行业标准SB/T10 2 78— 1997对其进行感官评定和水分测定及各组实验的出品率比较 .通过试验得出了 ,当 β 环糊精的使用量为鸭肉质量分数 0 .1%时 ,能有效除去鸭肉固有的腥味同时又不影响香肠的品质 ;β 环糊精含量过高香肠质量变差 ,而含量过低则不能有效的除去香肠中鸭肉的腥味

Abstract:Trace Pb in pure water was determined by GFAAS with multiple injections for concentration. Compared with conventional single injection, the precision was improved remarkably. The method was simple and fast. It was a useful method for analysis of trace me