Abstract:Starting with antibiotics, an introduction to strategy and means utilized for the improvement of secondary metabolites producing strains was given. Based on the theory of metabolic engineering, a further advances was made on the "five word strategy"whic

Abstract:Chitin/chitosan can be degraded not only by. chitinase, chitosanase and lysozyme but also by non specific enzymes such as papain, lipase, hemicellulase and so on. The reaction mechanism and kinetics of those degradations are discussed in detail, which i

Abstract:Preparation methods of noncrystallized granules of starch were systemically reported. They were high temperature swelling in water dispersing system and alkali swelling in NaOH solution. Changes from polycrystallization to noncrystallization were affirmed

Abstract:In order to obtain homogenous fractions, water soluble pentosans(WSP) and water insoluble pentosans(AEP) prepared from wheat bran were fractionated by stepwise ethanol precipitation and DEAE cellulose column chromatograph. The composition of each pentosa

Abstract:Candida glycerolgenses was mutated by NTG. The phosphorus concentration of screening medium, in which phosphorus source consists of corn steep liquor and inorganic phosphorus, was 460 mg/L. Several mutants were screened. Compared with original strain, t

Abstract:Base on the experimental data of batch fermentation in 30 L fermentation reactor, and the established mathematical model of L tryptophan fermentation, the optimal parameters were evaluated, and the nlinfit was fitted to the model through software MATLAB

Abstract:研究了培养温度、初始 pH、碳源及氮源等对隐甲藻 (CrypthecodiniumcohniiATCC3 0 5 5 6)悬浮培养生产DHA影响 .通过正交试验得到的优化培养基组成为 (g/L) :葡萄糖 2 0 ,甘油 2 0 ,蛋白胨 5 ,KNO3 5 ,NaCl6,MgSO41 .6,KH2 PO41 .0 ,VH 0 .0 0 6,VB12 0 .0 0 1 ,另外添加 1mL/L橄榄油 ,6mL/L丙酮酸 .优化培养条件为 :5 %接种量 ,初始 pH 7.0 .黑暗条件培养 ,装液量为 5 0 0mL三角瓶装 5 0mL培养基 ,3 0℃培养 3 2h后转到 2 0℃培养直至收获 .在优化培养基配方和培养条件下 ,隐甲藻悬浮培养 64h后 ,生物量和DHA产量分别为 1 0 .78g/L和 1 .2 1 g/L .

Abstract:Rhodotorula strain RY 3 9 of high yielding carotenoids was obtained by the technology of ion implantation. The carotenoids yield of this strain was increased by 76.2% compared with that of initial strain RY 3. Its genetic quality was proved stable by

Abstract:An improved pH stat fed batch culture strategy was developed for the effective production of glutathione synthetases from recombinant E.coli using a novel substrate feeding. pH was controlled by different pH controlling mode during batch culture and

Abstract:N-乙酰-D-葡糖胺是一种具有多种生理活性和功能性质的重要氨基糖 .实验研究了NAG的一些重要理化性质和加工稳定性 ,为NAG在食品中的应用提供理论依据 .结果表明 ,NAG易溶于水 ,微溶于乙醇 ;NAG晶体在 1 2 0℃、6h下保留率大于 98% ,性质稳定 ;在 1 0 0℃、6h、pH 3 .0～8.5的条件下 ,NAG水溶液稳定性好 ,pH超过 8.5则保留率大大降低 ,并伴有颜色变化 ;NAG在牛奶杀菌条件下性质稳定 ;NAG不具有吸湿性和保湿性

Abstract:为了保持茶多糖的活性 ,采用低温水提、酶提二次结合法提取茶多糖 .第一次在 5 0℃、茶叶与水的质量比为 1∶1 5、水提取 3 0min ,多糖的提取率为 2 .3 3 % ,粗多糖 (干重 )的提取率为6.82 % ;过滤后 ,滤渣用 pH 4.6的柠檬酸 柠檬酸钠缓冲液加纤维素酶提取 ,经正交试验确定酶提最佳工艺参数为 5 5℃、茶叶与水的质量比为 1∶1 4、酶用量 2 .2 μL/g(以茶叶质量计 ) ,反应时间为1 2 0min ,其多糖的提取率为 0 .64% ,粗多糖 (干重 )的提取率为 1 .1 1 % ,分别占二次总提取量的2 1 .5 %和 1 4.0 % ,而在相同条件下无酶提取 ,提取率仅为 0 .3 9%和 0 .5 6% .相对水提法 ,酶法的多糖提取率分别增加 63 .3 %和 98.9% ,多糖总提取率达 2 .97% ,粗多糖 (干重 )的总提取率为7.93 % .采用酶法提取的茶多糖具有较强抑制α 淀粉酶活力的能力 .

Abstract:The simulated moving bed (SMB) chromatographic technique has been used to separate the xylitol mother liquor into xylose and xylitol component. In the frame of equilibrium theory, characterized by purity and recovery, a new method is presented to study th

Abstract:A new technique was used to separation xylitol mother liquid with simulated moving bed (SMB) chromatography. The separation behavior and conditions on SMB chromatography were studied. The general law of separation xylitol mother liquid with SMB chromatogr

Abstract:采用正交试验探讨了微生物菌种、风速、相对湿度、风干时间、温度对风鸭风味及游离氨基酸产生的影响 .结果表明 ,这些因素对风鸭风味及游离氨基酸产生均有显著的影响 .其最佳风干条件为 ,菌种 :乳酸链球菌 +丁二酮链球菌 +微球菌 ;风速 :5m/s;相对湿度 :68% ;风干时间 :75h ;温度 :1 6℃ .

Abstract:The article is to establish a PCR method for detecting transgenic component 35S promoter derived from Cauliflower Mosaic Virus and NOS terminator derived from Agrobacterium tumefaciens simultaneously. According to the specific sequence of 35S and NOS

Abstract:This article mainly deals with a kind of novel photosensitive chitosan made from the mixture of photosensitive compounds and chitosan. Several series of photocrosslinked chitosan/poly vinyl alcohol (CTS/PVA) blend films were prepared and their struct

Abstract:In order to screening an optimum culturing medium of Agaricus blazei (AbM), single factor test in culturing utensil was carried out. The results showed that maize powder and sucrose were good carbon sources, and soybean powder and wheat bran extracts w

Abstract:The decoloration rate of methyl orange was reduced by 70% after 24 hours when 2 mmol/L ethylenediamine tetraacetic acid (EDTA) was added in fungal cultures of Phanerochaete chrysosporium adding .The decolorations of different dyes were reduced because

Abstract:The effects of a new type additives of chitosan on decreasing volatilizable aldehydes in cigarette smoke were investgated by adding into filter in cigarette and determining the content of volatilizable aldehydes by spectrophotemetric method. The influenc

Abstract:The mechanism of cloud stabilization for orange juice was investigated. The results indicated that the homogenization could enhanced the cloud stability of juice by decreasing the size of the particles. However, while homogenized at pressure higher than

Abstract:用酶法制取胡萝卜混汁 ,确定了最佳去皮工艺 (在 95℃下 4g/dLNaOH溶液中热处理1min)、热烫工艺 (在 95℃下 0 .6g/dL柠檬酸溶液中热烫 6min)、酶解工艺 (0 .0 0 3 5 g/dL果胶酶 ,0 .1 6g/dL纤维素酶 ) ,制得了具有一定营养价值、色泽鲜艳、混浊稳定性好、无不良胡萝卜本味的胡萝卜混汁

Abstract:This paper probes into the principles that phosphatides affect quality of peanut ice cream as emulsifiers. Fair strong poly molecular absorbing films were found in ice cream,and the films were that emulsifiers combined with molecules of protein,oil a

Abstract:Soybean isoflavones and saponins were extracted from soybean germs by 70% alcohol at room temperature, and determined using HPLC/ESI MS. According to their MS characters, nine kinds of isoflavones, seven types of group A saponins, four types of group B

Abstract:Coenzyme Q 10 was an important pharmaceutic functional material. The purity of the preparations of CoQ 10 was determined by TLC, UV and high performance liquid chromatography on the base of selected pretreatments of the fermentation culture wi

Abstract:Aspergillus ficuum fructanohydrolases were separated by native polyacrylamide gel electrophoresis. Eight protein bands were obtained. Proved using TLC and HPLC, three bands had exoinulinase activity and two bands had endoinulinase activity.