Abstract:In this study, 8 strains of lactic acid bacteria isolated from fermented foods were used to screen the strains producing conjugated linoleic acid (CLA) by ultraviolet spectrophotometry, and further evaluation of in vitro probiotic properties were conducted. The results showed that Pediococcus acidilactici ZFM801, Leuconostoc mesenteroides ZFM803, Lactiplantibacillus plantarum ZFM804, Lactiplanti-bacillus plantarum ZFM805, Lactiplantibacillus plantarum ZFM806, and Weissella cibaria ZFM810, had the ability to produce CLA, among which Lactiplantibacillus plantarum ZFM804 exhibited the highest CLA production capacity (mass concentration up to 21.7 mg/L) and consisted of c9,t11-CLA and t10,c12-CLA isomers. The results of prebiotic properties revealed that Lactiplantibacillus plantarum ZFM804 exhibited a high co-coagulation rate of 25% against three pathogenic bacteria. The tolerability study showed that Lactiplantibacillus plantarum ZFM804 could tolerate 4% mass fraction of NaCl and 0.1% mass fraction of bile salt, and Lactiplantibacillus plantarum ZFM804 also showed high tolerance under a simulate gastrointestinal fluid environment, with a survival rate over 60% at pH 4.5. Moreover, Lactiplantibacillus plantarum ZFM804 demonstrated a significant inhibitory effect on Micrococcus luteus and Staphylococcus aureus. In summary, Lactiplantibacillus plantarum ZFM804 possesses the potential for high CLA production and exhibits excellent prebiotic properties.
