Proliferation and Tolerance of Lactiplantibacillus rhamnosus Utilizing Different Prebiotics

Abstract:

To determine the optimal snybiotics of Lactiplantibacillus rhamnosus(LGG) and prebiotics, the LGG-galactooligosaccharide combination is significantly superior to other combinations through growth and pH changes. The maximum biomass OD₆₀₀ of LGG and HN001 were 1.253 and 1.552, respectively, and the maximum specific growth rates were 0.316 and 0.290 h^-1 when galacto- oligosaccharide was used as the sole carbon source. Similarly, the lactic acid yield also showed a significant preference for this combination over others, with yields of 3.654 and 10.914 g/L, respectively. Meanwhile, in order to confirm that the combination can successfully pass through the gastrointestinal tract and exhibit probiotic functions, the tolerance of LGG and HN001 under different pH and bile salt mass concentration was determined respectively when galacto-oligosaccharide was used as the sole carbon source. The results revealed a significant difference in the maximum biomass under 1 g/L bile salt and pH 4.0 conditions compared to other synbiotics, with OD₆₀₀ values of 0.712, 0.694 (1 g/L bile salt), 0.639, and 0.728 (pH 4.0), respectively. This study provides theoretical basis and experimental evidence for the subsequent development of related probiotic-prebiotic products.