Abstract:In order to explore a high-efficient method to promote the enzymatic hydrolysis of Procambarus clarkia with multi-mode ultrasound, the dual-frequency probe ultrasound and tri-frequency divergent ultrasound were used to pretreat Procambarus clarkii in this study, and the promotion effects of multi-mode ultrasonic pretreatment on the enzymatic hydrolysis of Procambarus clarkii were comparatively investigated. Through the single factor experiments, the optimal frequency mode of the dual-frequency probe ultrasonic equipment was found to be 20/28 kHz in the sequential mode, while that of the three-frequency divergent ultrasonic equipment was 20/40 kHz in the sequential mode. On this basis, the response surface experiment with three factors and three levels was carried out to optimize the ultrasonic pretreatment conditions and to establish a corresponding regression equation. The optimization experiment was conducted to obtain the best ultrasonic pretreatment conditions. The optimal pretreatment conditions of the dual-frequency probe ultrasonic equipment were determined, including the use of 250 W ultrasonic power, treatment time of 10 minutes, and temperature of 35 ℃. As a result, the degree of hydrolysis of Procambarus clarkii reached 38.98%. Similarly, the tri-frequency ultrasonic equipment achieved an optimal hydrolysis rate of 46.12% under the optimal conditions of 250 W ultrasonic power, 40 minutes duration, and 40 ℃. The experimental results showed that multi-mode ultrasound could effectively improve the enzymatic hydrolysis effect of Procambarus clarkii.
