Abstract:A hypertensive rat model was established using a high-salt diet. Based on high-throughput sequencing technology, the potential injury mechanism of hypertensive target organs (ventricle and atrium, renal cortex and medulla, colon) and the effect of a high-salt diet on the intestinal microflora were explored. The results showed that rats exhibited a significant increase in blood pressure after 12 weeks of a high-salt diet. A series of dysregulated differential transcription factors were found in the transcriptome data analysis of target organs affected by hypertension. 16S rRNA sequencing results showed that the composition of intestinal microflora in rats was changed. The functional analysis revealed a close association between these changes and microbiome metabolic dysfunction. Co-expression analysis further explored the potential mechanisms of crosstalk between the genome and microbiome in the development of hypertension. In conclusion, this study provides candidate key genes and the intestinal microbiome associated with the treatment of high-salt diet-induced hypertension and heart-kidney-intestinal injury. Innovative multi-omics analysis may be a crucial approach to understanding gene expression patterns and disease development.
