Abstract:Deoxynivalenol (DON), also known as vomitoxin, is produced by Fusarium graminearum. It is neurotoxic, immunotoxic, embryotoxic, teratogenic, and carcinogenic. DON mainly contaminates wheat, which not only leads to the quality deterioration of wheat but also jeopardizes the health of human beings directly or indirectly. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and gold immunochromatographic assay (GICA) were employed to determine the mass fraction of DON in wheat. The accuracy and stability of the two methods were evaluated by the spiked recovery test, and the recovery of the two methods was 92.98%~97.43% and 104.53%~108.92%, respectively, with the relative standard deviations (RSD) less than 10%. Then, the two methods were used to determine the mass fraction of DON in each part of naturally polluted wheat after processing. After milling and processing, the mass fraction ratio of DON in flour to whole wheat flour averaged 0.56, and that of DON in bran to whole wheat flour averaged 1.55. The results showed that the mass fraction of DON in flour was significantly lower than that in whole wheat flour after milling and processing of wheat, while the mass fraction of DON in bran was significantly higher than that in whole wheat flour. Therefore, the separation of bran from wheat after processing to the maximal extent can effectively reduce the mass fraction of DON in flour.
