Abstract:To improve the biological activity of food flavone isoorientin (Iso) and provide a basis for preventing liver injury induced by benzo (a) pyrene (BaP), Iso and its silver nanoparticles (Iso-AgNPs) were used to investigate to the damage of HL-7702 human liver cells caused by BaP, a harmful product of food prepared under high temperature. The hepatoprotective effect of Iso-AgNPs was detected by MTT, trypan blue staining, fluorescence labeling, flow cytometry, and Western blotting based on the three programmed death modes including apoptosis, autophagy and pyroptosis. The results showed that compared with Iso, Iso-AgNPs could better inhibit the damage of HL-7702 huamn liver cells activity induced by BaP, promote cell proliferation, and significantly inhibit the apoptosis, autophagy and apoptosis of HL-7702 huamn liver cells. Therefore, Iso-AgNPs could improve the biological activity of Iso and prevent liver cell damage.
