Research Progress of Sucrose Isomerase in Isomaltulose Production

Abstract:

Isomaltulose is a new multifunctional sweetener and an internationally recognized safe sucrose substitute. Isomaltulose has wide application prospects in the medicine and food industries. Sucrose isomerase (SIase) is the most effective biological enzyme reagent for the production of isomaltulose by biological transformation. This review briefly presents the physiological characteristics of isomaltulose as well as the existing problems in its production. The source, three-dimensional structure, catalysis mechanism, rational design of high efficient enzymes and heterologous efficient expression of SIase were discussed in detail. In addition, the wide application of cell and enzyme immobilization in the production of isomaltulose was then introduced. Finally, the application of SIase in isomaltulose production was prospected.