Abstract:[Objective] This study aims to investigate the in vivo and in vitro antioxidant activities of Agaricus bitorquis (Quél.) Sacc. polysaccharides, including polysaccharides from sporocarp (PS), intracellular polysaccharides (IPS), and exopolysaccharides (EPS). [Method] Five in vitro antioxidant indexes and four in vivo antioxidant indexes were determined using an in vitro antioxidant system and an in vivo antioxidant (mice injured by hypoxia oxidation) model. [Result] The results showed that IPS, EPS, and PS all had scavenging abilities against DPPH radical, ABTS radical, hydroxyl radical, and superoxide anion radical but at significantly different levels (P<0.05), and the effect of IPS was the best. IPS, EPS, and PS could decrease the concentration of malondialdehyde (MDA) and increase the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in the blood of mice injured by hypoxia oxidation, and the levels changed by the three kinds of polysaccharides were significantly different (P<0.05). Compared with the blank group, IPS could decrease the concentration of MDA by 73.00% and increase the activity of CAT by 28.93%. The activities of SOD and GSH-Px were increased by 25.08% and 72.14%, respectively by PS. [Conclusion] All the three kinds of polysaccharides from Agaricus bitorquis (Quél.) Sacc. have strong antioxidant activity in vivo and in vitro, with IPS having the highest antioxidant activity, which has high research value as a natural antioxidant.
