Abstract:In this study, the silicon carbon quantum dots (SiCDs) with a specific response to acetamiprid pesticides were modified and prepared, which were then used as the fluorescence probe to develop a highly sensitive molecularly imprintied fluorescence sensor (SiCDs@MIPs) to rapidly detect the acetamiprid pesticides. The effects of the ratio of template to functional monomer, the amount of cross-linking agent, and the amount of SiCDs on the detection sensitivity of SiCDs@MIPs were further investigated. The results showed that the optimal preparation conditions of SiCDs@MIPs were as follows: the ratio of template to functional monomer was 1∶4, the amount of cross-linking agent was 25 μL, and the embedding amount of SiCDs was 10 mg. Under the optimal detection conditions, the detection limit of SiCDs@MIPs toward acetamiprid was 0.086 μmol/L within the linear range of 0.045 to 0.450 μmol/L, the correlation coefficient was 0.989, and the detection time was only 25 minutes. When SiCDs@MIPs was further applied to determine the acetamiprid pesticide residues in Chinese cabbage, tomato and wax gourd, the recoveries ranged from 73.0% to 109.0%. The proposed method provided a new strategy for the determination of acetamiprid pesticide residues in vegetables.
