Abstract:The use of microfluidic chips for polymerase chain reaction (PCR) can improve detection efficiency and automation. However, it is still difficult to integrate the nucleic acid extraction, amplification and detection functions into one chip. In addition, the temperature control of the special PCR instrument for microfluidic chip is not fast and accurate enough. Based on the principle of temperature cycling in space instead of temperature cycling in time, a rotary three-temperature zone microfluidic PCR amplification platform was built by the authors to extract and amplify E. coli nucleic acids. The results show that the rotary three-temperature zone microfluidic PCR amplification platform has good thermal uniformity and stability. The heating and cooling rates of the platform were 3.5 ℃/s and 2.67 ℃/s, respectively. The single cycle time is only 110 s. Compared with the 9700 PCR, the platform has a simpler temperature control scheme, higher heating and cooling rates and shorter cycle ti
