Metabolic Regulation and Synthesis of Functional Nutrients in Saccharomyces cerevisiae: Progress and Challenges

Abstract:

The research progress of Saccharomyces cerevisiae as a chassis cell for metabolic regulation and synthesis of functional nutrients was summarized in this review. Firstly, a series of metabolic regulation strategies related to the promoter regulation, terminator regulation, cofactor regulation, transporter regulation and dynamic regulation of Saccharomyces cerevisiae as a cell factory were introduced, and the related genetic transformation tools and metabolic analysis techniques to enhance product synthesis were also introduced. Secondly, the research progress on synthesis of functional nutrients, such as oligosaccharides, vitamins and terpenoids, short chain organic acids and fatty acids, by Saccharomyces cerevisiae was summarized. Lastly, the current challenges were put forward, and the future development trend of metabolic regulation of Saccharomyces cerevisiae was prospected in combination with the emerging technologies of metabolic engineering, in order to provide a reference for the efficient synthesis of functional nutrients in Saccharomyces cerevisiae.