Abstract:The effect of ionic liquid pretreatment on the enzyme catalytic activity was investigated to improve the synthesis efficiency of phosphatidyl-N-acetylglucosamine (PtdGlcNAc) and different imidazole ionic liquids were selected to study the transphosphatidylation of phosphatidylcholine (PC) and N-acetyl-beta-D-glucosamine (GlcNAc) catalyzed by phospholipase D (PLD). The results showed that the enzyme activity of PLD pretreated with 1-butyl-3-methylimidazole hexafluorophosphate ([BMIm][PF6]) was significantly higher than that of other ionic liquids and untreated blank groups. Therefore, [BMIm][PF6] was used as the incubation solution for PLD pretreatment. Through the optimization experiment, the optimum reaction conditions were as follows: 1 U PLD, the amount ratio of substrate PC to GlcNAc of 1∶60, cyclopentyl methyl ether as the organic phase, and the volume ratio of ionic liquid phase to aqueous phase of 4∶1. After 12 h, the product yield was 79.7%, which was 56.9% higher than that of the untreated one. PtdGlcNAc were synthesized into nanoliposomes which structures were preliminary characterized. This study could enrich the related research of PLD and provide a reference for the synthesis and application of other novel phosphatidyl compounds.
