Effects of Fermentation Conditions on Mycelial Pellet Morphology of Aspergillus terreus and Aflatoxin B1 Degradation Efficency

Abstract:

[Objective] To study the effects of different fermentation conditions on the mycelial pellet morphology of Aspergillus terreus (A. terreus) AterHaut1 and aflatoxin B₁ (AFB₁) degradation efficency. [Method] Single factor experiments were carried out to investigate the mycelial pellet morphology and AFB₁ degradation rates under different inoculation volume fractions, fermentation temperatures, and shaker speeds. The fermentation conditions were optimized by Box-Behnken design combined with response surface methodology. [Result] The optimal fermentation conditions were obtained as follows: shaker speed of 145 r/min, inoculation volume fraction of 0.17%, and fermentation temperature of 35 ℃. The actual degradation rate of AFB₁ by the fermentation supernatant obtained under the optimal fermentation conditions reached 96.34%. At this time, the average diameter of mycelial pellets was 1.35 mm, with even distribution between 1.20 mm and 1.50 mm. [Conclusion] Optimizing the fermentation conditions of A. terreus AterHaut1 can improve the degradation rate of AFB₁.