Abstract:In order to solve the hidden danger of food safety, gas chromatography-mass spectrometry (GC-MS) combined with headspace solid phase microextraction (HS/SPME) was constructed to explore the volatile metabolites of Staphylococcus aureus, and the different metabolites and metabolic pathways of Staphylococcus aureus in milk were analyzed by the combination of metabonomics and genomics. The results showed that the different metabolites of Staphylococcus aureus included N-methylthioformamide, 1-butanol, acetic acid, ethylbenzene, mesitylene, octanoic acid, benzoic acid, n-decanoic acid, dodecanoic acid, ethanol, phenol and propylene glycol. After genomic analysis, Staphylococcus aureus was mainly based on the basic life metabolism, and the metabolic pathways and related genes of the substances involved in metabolism (ethanol, acetic acid, 1-butanol) in the metabolic network were identified. This study could provide a theoretical basis for the study of biomarkers of Staphylococcus aureus.
