Preliminary Study on Relationship between Antimicrobial and Antioxidant Spectra of Isaria cicadae Miquel

Abstract:

This study aimed to establish the bacteriostasis and antioxidant relationship among different parts of artificially cultivated Isaria cicadae Miquel (IcM) and screen the active components. Ultra-performance liquid chromatography (UPLC) fingerprints of different parts of IcM were established, and in vitro antibacterial and DPPH antioxidant activity were performed on the extracts of spore powder and sterigmata. The pharmacodynamic data was correlated with the common peaks of chromatographic fingerprints by the cluster analysis and grey relational analysis to establish the spectrum-activity relationship. The extracts from different parts of IcM were effective in inhibiting Staphyloccocus aureus and scavenging free radicals. The extract from IcM spore powder showed better therapeutic effect than that form sterigmata. Grey relational analysis showed that the bacteriostasis and antioxidant activities of IcM were the combined effects of various components, and the characteristic peaks were No.23, No.2, guanosine and adenosine (correlation degree>0.85), which greatly contributed to the pharmacodynamics. The preliminary spectrum-activity relationship of different parts of IcM was established in this study, and the characteristic peaks which greatly contributed to the pharmacodynamics of IcM were screened out, providing reference for the further study of the pharmacodynamics and pharmacology of IcM.