Abstract:Hot water extraction was used to extract bee pollen crude polysaccharides to investigate the effect of different extraction methods on the antioxidant activities of bee pollen polysaccharides. The effect of temperature and ethanol volume fraction on the extraction rate of bee pollen polysaccharide from three kinds of pollenwere studied. The scavenging effect of bee pollen crude polysaccharides towards superoxide and hydroxyl radical was detected under pyrogallol autoxidation and fenton oxidation, respectively. At last, the effect of pollen polysaccharides on myofibrillar protein oxidation was explored by measuring the content of protein carbonyl group (molar concentration) in the flesh of acrolein-treated large yellow croaker (Pseudosciaenacrocea). The results indicated that the antioxidant effect of camellia crude polysaccharides extracted at 80℃ was most significant against superoxide radicals. The scavenging effect of ethanol volume fraction on superoxide free radicals of three kinds of pollen polysaccharides was significant, but the scavenging effect onhydroxyl radicals was insignificant. Pollen polysaccharides extracted at 80 ℃ and 95% ethanol concentration significantly reduced the carbonyl content and increased total sulfhydryl content in the large yellow croaker myofibrillar protein treated with acrolein. The effect of crude polysaccharides from wild chrysanthemum pollen was the best. It was concluded that the antioxidant activity of bee pollen was affected by extraction temperature and ethanol volume fraction, and bee pollen polysaccharides couldinhibit protein oxidation, among which wild chrysanthemum pollen polysaccharides showed the best antioxidant effect.
