Progress of Loop-Mediated Isothermal Amplification in Detection of Cronobacter sakazakii

Abstract:

Cronobacter sakazakii is a kind of foodborne pathogenic bacteria, which can cause neonatal meningitis, septicemia and other diseases. Loop-mediated isothermal amplification technique (LAMP) is a new type of isothermal nucleic acid amplification technique with high sensitivity, short time-consuming, high specificity and low equipment requirements. It can be combined with a variety of detection methods for the detection of foodborne pathogenic microorganisms. This paper aims to elucidatethe basic principles of LAMP and summarize the progress of LAMP technology combined with different detection methods in the detection of C. sakazakii.