Abstract:In order to screena strain that could effectively degrade ciprofloxacinand to explore its degradation characteristics in the degradation process, high performance liquid chromatography was employed to screen ciprofloxacin degrading bacteria. This bacterium was then identified according to the colony morphology, physiological and biochemical characteristics as well as the 16S rDNA gene sequence analysis. The strain activity, and the degradation of intracellular and extracellular substances and cell walls on ciprofloxacin were also investigated with 4 μg/mL ciprofloxacin. The results showed that the degradation rate of ciprofloxacin by the screened Lactobacillus reuteri WQ-Y1 strain reached to 70.2% via physical adsorption and biodegradation. Furthermore, the degradation rates of ciprofloxacin by intracellular substances, extracellular substances and cell walls were 17.7%, 23.4% and 37.0%, respectively, which were far lower than that of the living bacteria. It could be concluded that the physical adsorption and the enzymolysis process of L. reuteri WQ-Y1 were not the only way of ciprofloxacin degradation, and it was also dependenton the complete liability of L. reuteri WQ-Y1.
