Abstract:Based on the characteristics of aroma production and promotion of laccase synthesis, a carotenoid-producing strain of Rhodotorula mucilaginosa YG14 was screened from tobacco stems. After solid-state fermentation of Ganoderma lucidum, Rhodotorula mucilaginosa YG14 and co-cultured mixed stalks. The contents of cell wall materials were measured, the neutral aroma components were determined, and the sensory quality evaluation was performed on the stalks. The results showed that the solid fermentation fermented silk of the two strains co-cultured had significant improvements in cell wall material degradation, neutral aroma content, and sensory absorption compared to those of two strains alone. After solid-state fermentation of the co-cultured mixed bacteria, the total cell wall material of stems degraded to 37.20%, and the neutral aroma components content (mass fraction) increased by about 20.44%. The sensory evaluation showed that the sweetness was significantly improved, the woody flavor was significantly improved, the aroma was improved to a certain extent, the burning sensation was significantly improved, and the aroma was more mellow. It can significantly improve the quality of tobacco stems and increase its utilization rate in tobacco.
