Analysis of the Differences of Metabolites between Lycium ruthenicum Murr. and Lycium barbarum L. by LC-MS

Abstract:

This study aimed to analyze and compare the differences of metabolites between Lycium ruthenicum< Murr. and Lycium barbarum L.. Liquid chromatography mass spectrometry technique (LC-MS) was adopted to analyze the metabolites. A total of 6 982 metabolites were detected, including 2 704 negative ions and 4 278 positive ions. Principle component analysis (PCA) displayed that Lycium ruthenicum Murr. and Lycium barbarum L. could be distinguished. Analysis of differential metabolites revealed a total of 501 differential metabolites. Among the top 50 differentially expressed metabolites, 7 were flavonoids, and 6 were amino acids and polypeptides. 11 metabolic pathways were significantly enriched, 4 of which were associated with amino acid metabolism. This study provided a basis for in-depth understanding of the biosynthesis of amino acid metabolism and the differential utilization of Lycium ruthenicum Murr. and Lycium barbarum L..