Abstract:This study mainly analyzed the components of sea cucumber ovum and studied their effects on the cognitive discrimination in mice. 70 SPF (Specific Pathogen Free) male ICR (Institute of Cancer Research) mice were randomly divided into the control group, scopolamine group, piracetam positive group (400 mg/kg), sea cucumber ovum low, medium and high dose groups (75, 150 and 450 mg/kg). Mice were intragastrically administered 30 minutes before intraperitoneal injection of 1 mg/kg scopolamine daily. After 30 days, mice were subjected to different behavioral experiments including open field test, new object recognition test, Y maze, Morris water maze, and step-through task. During the behavioral experimental training period, oral administration and scopolamine injection were continued to establish the model. The results showed that compared with the scopolamine group, the sea cucumber ovum group significantly increased the active time of mice in the central area of open field (P <0.05), and significantly improved their discrimination index in the object recognition test (P <0.05). Spontaneous alternation was significantly increased in the Y maze P <0.01), and the escape latency was significantly reduced in the Morris water maze space exploration test(P <0.01). At the same time, it significantly increased the crossing times (P< 0.05) and cumulative time(P < 0.05) of mice in the platform area. During the dark avoidance experiment, the latency of mice entering the dark roP om was significantly increased(P < 0.05) and the number of errors was significantly decreased (P < 0.05).
