Abstract:A visual detection of zearalenone in food by competitive enzyme-linked aptamer assay was developed. A biotinylated zearalenone aptamer was immobilized on the surface of microplate wells by biotin-avidin binding. The zearalenone aptamer's complementary(cDNA) was ligated with horseradish peroxidase (HRP) to form a cDNA-HRP signal probe. Then the cDNA-HRP signal probe and target competitively combined with the aptamer. The visual detection of zearalenone could be achieved after the addition of TMB chromogenic reagent and stop solution(sulfuric acid). Under the optimized conditions, the detection limit was 0.7 ng/mL and the linearity was good in the range of 1 to 10 000 ng/mL(R2=0.991 3). Compared with other similar mycotoxins, the developed method had good specificity to zearalenone. And the spike recoveries of beer and corn samples were 88.57%～102.14% and 91.43%～106.43%, respectively. The method has high sensitivity, good specificity and low detection cost, which is suitable for the visual detection of zearalenone in food.
