Abstract:This study was to explore the effects of heat shock protein A1A(HSPA1A) on the proliferation of HeLa, HT29 and PC3 tumor cells and their underlying mechanisms. HSPA1A recombinant vector was constructed to transfect tumor cells. The cell morphology, MTT and cell cycle after transfection were observed to explore the effect of HSPA1A on tumor cell proliferation. The cells that HSPA1A played a role in cancer cell proliferation were determined by immunofluorescence positioning. After overexpression of HSPA1A, cell proliferation of tumor cells was significantly enhanced. Cellular immunofluorescence confirmed that HSPA1A was mainly localized in the cytoplasm. Cell cycle assay showed that transfection of HSPA1A mainly accelerated the G1 phase of cell growth cycle, which was determined by Western Blot. Overexpression of HSPA1A up-regulated G1-related cyclin D1, CDK4, CDK6, cyclin E1, and CDK2. It was speculated that HSPA1A might serve as a chaperone protein to maintain the stability of cyclin D1-CDK4/CDK6 and cyclin E1-CDK2-related proteins and accelerate the G1 phase of cell cycle. HSPA1A can promote tumor cell proliferation and play a role in the cytoplasm proliferation. This study provides a theoretical basis for the development of anti-tumor drugs based on HSPA1A.
