Co-Catalysis of Deoxyribose Aldolaseand Alcohol Dehydrogenase to Generate (3R,5S)-6-Chloro-2,4,6-Trideoxy-Erythro-Hexonolactone

Abstract:

The biological coupling method was used in tandem with the enzyme reaction process to synthesize (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexonolactone, the statin side-chain intermediate, in 'one-step' bio-synthesis. The recombinant plasmids of Streptococcus suis deoxyribose aldolase gene and Lodderomyces elongisporus alcohol dehydrogenase gene were co-transformedinto the same cell of Escherichia coli BL21 to realize their soluble co-expression.(3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexonolactone was obtained by the whole cell co-catalysis of 400 mmol/L acetaldehyde and 200 mmol/L chloroacetaldehyde using recombinant E. coli was used as the biocatalysts. In the absence of standard products, mass spectrometry and 1H nuclear magnetic resonance were performed to identifythe products. The final product was determined as the statin side-chain intermediate (3R,5S)-6-chloro-2,4,6-trideoxy-erythro-hexonolactone.