Detection of Crocodile Originin Meat by Fluorescence Quantitative PCR

Abstract:

A fast, specific and sensitive method for detection of crocodile-derived components was developed by designing specific primers on the cytb gene sequence of crocodiles. Mutton, beef, pork, chicken, duck, fish and shrimp were used as reference animals for specific detection. The feasibility of the method was verified by the detection limit and sensitivity test. The results showed that the method was effective in rapid detection of crocodile-derived components, with strong specificity, and the detection limit of DNA of crocodile-derived components was up to 0.001 ng/μL, and the sensitivity was up to 0.01%. The method has the characteristics of strong specificity and high sensitivity, which can be used for the detection of crocodile-derived components in processed meat products.