Abstract:Pseudomonas putida is one of the most important industrial microorganisms, widely applied in environmental remediation, production of bioactive natural products and secondary metabolites, etc. To enhance the n-butanol tolerance of Pseudomonas putida 901, atmospheric and room temperature plasma(ARTP) was performed to generate mutant library. After gradient adaptation in n-butanol, a mutant capable of tolerating 12 g/L n-butanol was obtained. The whole genome was re-sequenced and analyzed through Illumina HiSeq platform. About 19 million reads was obtained with an average read of 150 nt. Compared with the reference genome, the fragment pairing rate was 92.03%,while the coverage rate was 90.34%. A total of 40 704 single nucleotide variations was detected, with isozygoty of 39 319 and heterozygote of 40 704. There were 2 153 inserts and deletions, with homozygous mutation sites of 1 871 and heterozygous mutation sites of 282. All the mutations were functionally annotated according to GO/KEGG database.
