Preparation of High Sesaminol Sesame Oil by Aqueous Enzymatic Method

Abstract:

To prepare high sesaminol sesame oil, a screening was performed to select an enzyme having the strongest ability to yield sesaminol by aqueous enzymatic method. Based on single factor test and response surface methodology, the optimal aqueous enzymatic extraction conditions of high sesaminol sesame oil were obtained as following: Aromase H2, reaction temperature 55 ℃, pH 4.5, time 9.2 h, enzyme dosage 1% and the ratio of solid to liquid 1∶1.3. Under the optimized condition, the content of sesaminol in sesame oil was increased up to 3 000 mg/kg. The total amount of lignans was increased by nearly 23%.