Abstract:In order to study the effects of different separation sources on the genome and function, 33 Lactobacillus plantarum from fermented sauce, kimchi and feces were selected. The basic characteristics of the genome, orthologous genes and system evolutionary relationshipwere studied by comparative genomics, combined with functional gene annotation results and phenotypic results to analyze strain resistance to antibiotics. The phylogenetic tree revealed a significant effect of the separation source on the genetic evolution of Lactobacillus plantarum. The homologous gene investigation showed that the fecal source strain had a higher specific gene number thanothers. 33 strains of Lactobacillus plantarum contain resistance genes of ciprofloxacin, tetracycline, chloramphenicol, trimethoprim and vancomycin, as well as their antibiotics. Most strains are sensitive to streptomycin, streptomycin, kanamycin, neomycinand ampicillin, and no related resistance genes were found in the genome. The genes and phenotypes of erythromycin and clindamycin do not correspond. The antibiotic experiments indicated an insignificant effect of the separation source on the strains and most of the genotypes and correspondence to the phenotype. Genomics is instructive to study the physiological characteristics of Lactobacillus plantarum.
