High Sensitivity Analysis of Kanamycin Based on Signal Amplification of Carbon Nanotubes

Abstract:

As one of aminoglycoside antibiotics，kanamycin has been widely used in clinical treatment and animal health because of broad spectrum antimicrobial activity，which leads to its widespread residues in animal-derived foods. Therefore，it is necessary to detect kanamycin residues in food. In this study，using multiwalled carbon nanotubes（MWCNT） as carriers，monoclonal antibody against kanamycin and horseradish peroxidase（HRP） were conjugated with MWCNT by EDC/NHS methods. A direct competitive ELISA based on MWCNT-Abs-HRP was constructed and itssensitivity was 5 times higher than that of the conventional competitive ELISA. The concentrations of kanamycin in six bovine milk from local market were evaluated.