Aptamer-Based Lateral Flow Strip for Rapid Detection of Listeria monocytogenes

Abstract:

An aptamer-based lateral flow strip was developed for the rapid detection of Listeria monocytogenes. This assay was designed based on the sandwich-type format by using primary aptamer conjugated with gold nanoparticles（AuNP） as a signaling probe and secondary aptamer coated membrane as a capturing probe. Several experimental conditions were investigated，including the concentration of aptamer，pH of AuNP solution，and loading quantity of AuNP-aptamer conjugates and streptavidin-aptamer conjugates. Under optimized conditions，the aptamer-based lateral flow strip showed a visual limit of detection（LOD） of 103 cfu/mL，and had no significant cross-reactivity with several food-borne pathogens. The aptamer-based lateral flow strip was shown to provide a reliable，accurate method for rapid detection of Listeria monocytogenes in the real samples.