Comparison of the Structural Characteristicsand Biological Activity of before and after Purification olysaccharides of L entinula edodes

Abstract:

The effect of purification on the structure and biological activity of polysaccharide in Lentinula edodes were investigated. The structural characteristics（UV，FT-IR，SEM，Viscosity- average molecular weight，Congo red complex）and biological activity（Antioxidant and Antibacterial activity）of crude polysaccharide samples （named L0）and their purified three polysaccharides （named L1，L2，and L3）were measured respectively. The UV results showed that nucleic acids and proteins were not found in the purified polysaccharides. The FT-IR results showd that all components have absorption peak of sugar and β-glucosidic bond，but the FT-IR structural of L1 were similar to L0. The SEM pictures showd that the surface structures of L2 and L3 were similar，presents the fragmental accumulation，and the structure of L1 present a bump and fold block. With Congo red complex of the study，L0 and L1 contained three spiral structure，the L2 and L3 did not contain three spiral structure. The antioxidation experiment showed that the L0 components had better anti-oxidation activity. The determination of staphylococcus aureus was found that L1 have better antibacterial activity. The work found that after purification，the structure and biological activity of polysaccharide were different. These provided some basis for the selection of active components.