Immobilization of Candida rugosa lipase for the Synthesis of Geranyl Butyrate

Abstract:

This study focused on the immobilization of Candida rugosa lipase（CRL） onto four kinds of different resins by adsorption. The Bradford method was adopted to determine the immobilization ratio，and GC method was used to measure esterification ratio of geranyl butyrate. Finally，the weak-acidic macroporous cation exchange resin D151 obtained the best results of immobilization. Taking the influence of factors including enzyme concentration，the pH of phosphate buffer and the temperature into consideration，the esterification ratio of geranyl butyrate production catalyzed by the immobilized CRL was set as evaluation index to optimize the immobilization process. The results indicated that the highest esterification ratio of 84% could be achieved by the CRL immobilized on D151 resin under optimized conditions：15 mg/mLCRL ，pH of the phosphate buffer 6.5，and immobilization temperature 45 ℃。