Constrcution and Comparison of Anti-AFB1 ScFv from Different Hybridoma Cell Lines

Abstract:

Anti-AFB1 Monoclonal antibody secreted by the differeent hybridoma cell lines have different sensitivity，three different hybridoma cell lines named 2C10，2E6 and 2H1 were screened and studied，the result of enzyme-linked immunosorbent（ELISA） test showed that the sequence of the sensitivity of monoclonal antibodies secreted by 2C10 ，2E6 and 2H1 was 2C10>2E6>2H1. Three anti-AFB1 scFvs from different hybridoma cell lines were constructed and expressed in E. coli BL21（DE3） respectively in order to better show and understanding the relationship between antigen and antibody and improve the sensitivity of recombinant antibodies at the greatest extent. The ELISA method was used to detect the sensitivity relationship between the different monoclonal antibodies and anti-AFB1 scFvs after protein expression and purification. The result showed that the sequence of the sensitivity of anti-AFB1 scFvs was scFv-2C10>scFv-2E6>scFv-2H1，and showed that the sensitivity of monoclonal antibodies were much better than the corresponding anti-AFB1 scFvs.