Effects of Overexpression of α-PGM on Ganoderma lucidum Polysaccharide Fermentation

Abstract:

α-Phosphogluconate，an important enzyme in polysaccharide synthesis pathway in Ganaderma lucidum，was overexpressed to determine polysaccharide production in submerged fermentation. In this study，the homologous pgm gene was transformed into G.lucidum protoplasts by Agrobacterium tumefaciens mediated method. The intracellular polysaccharides（IPS） and extracellular polysaccharides（EPS） production of G.lucidum transformant was 21.02 mg/100 mg mycelium and 0.71 g/L，respectively. The overexpression of pgmin G. lucidum effectively enhanced IPS（9.1%） and EPS（39.2%） production comparing with wild strain. Moreover，the transcription levels of pgm and pgi gene in polysaccharide synthesis pathway were up-regulated，and intracellular activities of these two enzymes were also significantly enhanced in comparison to wild strain.