Study of Preparation Process of Tomato Powder Rich in Amadori Compounds by UPLC-MS/MS

Abstract:

To prepare tomato powder rich in Amadori compounds. A UPLC-MS/MS method using caffeine as an internal standard was used to detect and quantify Amadori compounds by optimizing the extraction method of Amadori compounds. Besides，the influence of water activity，heating temperature and time，concentration of histidine and antioxidants on the yield of Amadori compounds were studied. The optimum extraction conditions were as follows：extract solvent water and extraction time 10 min；The optimal heating conditions were as follow：raw material tomato powder with water activity as 0.44 with the addition of 0.048 mg/g tea polyphenol，2.8 mg/g histidine，heating at 54 ℃ for 4.2 h. This method is simply and accurate. The concentration of Amadori compounds and Fru-His in the prepared product was respectively 152.59 mg/g and 3.32 mg/g，which were larger than any other tomato products.