Inhibition of Streptococcus mutans and Its Biofilm Formation by Lactobacillus plantarum FB-T9

Abstract:

In order to screen probiotics that had a inhibitory effect on Streptococcus mutans and its biofilm formation，lactic acid bacteria were isolated from oral samples and feces of healthy people and fresh vegetable leaves，among which 12 strains that could significantly inhibit the growth of S. mutans were selected to detect their abilities of inhibiting the biofilm formation of S. mutans. The abilities of auto-aggregation，co-aggregation，self-biofilm formation，surface hydrophobicity and surface acid-base charge of these strains were tested，and finally Lactobacillus plantarum FB-T9 was selected by PCA analysis. The inhibition of biofilm formation of S. mutans by FB-T9 at different time was further studied. To investigate the survival adaptation of FB-T9 in oral environment，the adhesive capacity to saliva-coated hydroxyapatite and lysozyme tolerance of FB-T9 were measured. L. plantarum could strongly inhibit the growth of S. mutans and its biofilm formation and showed ideal characters. The adhesive capacity of this strain was 32.93% and the maximum tolerated concentration of lysozyme was 1.2 mg/mL. The results showed that L. plantarum FB-T9 could be a potential probiotic to prevent dental caries.