Absorption and Distribution of Soybean Meal Protein in Mice Gastrointestinal Tract

Abstract:

The absorption and distribution of soybean meal protein in mice gastrointestinal tract in different time periods after single dose of soybean meal were investigated. In this study，the protein in soybean meal was used as a marker，to study the absorption of soybean meal in the gastrointestinal tract of mice. Amount of soybean meal at each part of the gastrointestinal tract was quantified by double antibody sandwich enzyme linked immunosorbent assay. The distribution of soybean protein in the gastrointestinal tract was located by immunogold silver staining. The results showed that the soybean meal protein mainly distributed in the small intestine after 0.5—1 h uptake，some in the stomach，and a few soybean meal protein was found in the large intestine，and after 2—4 h，the soybean meal protein distribution tends to be stable，which was consistent with the absorption positions and pathway of protein. Furthermore，using a stable food protein as a marker could be a new idea for studying the absorption and distribution of the food in the gastrointestinal tract.