Optimization of Two-Step Enzymatic Degradation of Polyvinyl Alcohol

Abstract:

As one of the most important pollutants in textile and paper industries，polyvinyl alcohol（PVA） has caused much attention. To improve the degradation efficiency，the reaction conditions of PVA degradation by using polyvinyl alcohol dehydrogenase（PVADH） and oxidized polyvinyl alcohol hydrolase（OPH） were optimized. Based on the Box-Behnken design and response surface strategy，optimal conditions were set as follows：PVA 1 g/L，PVADH 123 U/mL，OPH 12 U/mL，pH 7.7，reaction temperature at 41 ℃，Ca2+ 1 mmol/L，PQQ 6 μmol/L. Under these conditions，the degradation efficiency can reach over 95% after PVADH and OPH 4 h reaction. These results indicated that PVADH and OPH can co-degrade PVA efficiently，which will promote industrial applications of enzymatic degradation of PVA.