Antibacterial Activities and Stabilities of Polysaccharide from Flowers and Leaves of Female Plant in Industrial Hemp

Abstract:

Polysaccharide from flowers and leaves of female plant in industrial hemp（PFLFIH） was extracted by decoction and alcohol sedimentation technique. Its antimicrobial activities in vitro of 9 kinds of normal pathogenic bacteria and fungi were determined by comparing the size of K-B inhibition zone. The effects of ultraviolet rays，temperature，solvents pH value，oxidant and reducer on its antimicrobial activities were also discussed. The results showed that PFLFIH had a good bactericidal effect on Staphylococcus aureas. Its minimum inhibitory concentration（MIC） was 3.125 mg/mL and minimum bactericidal concentration（MBC） was 6.25 mg/mL. Meanwhile，it has no antimicrobial activities of other 8 pathogenic bacteria and fungi. High temperature，ultraviolet rays，oxidant and reducer treatments did not affect its inhibitory effect. While pH value of the solvent environment is 7，the best antimicrobial activity could be shown.