Extraction Total Flavonoids and Determination of Main Compounds in Kushui Rose

Abstract:

Surface response optimized and extraction total flavonoids and determination of main compounds in kushui rose，under this condition，using Ultra Performance Convergence Chromatography（UPC2） to determined quercetin and kaempferol in total flavonoids. The parameters of extraction technology of total flavonoids from kushui rose were optimized by four factors and three levels response methodology using ethanol concentration，issue-liquid ratio，extraction time and extraction temperature as the response factors and with yeild rate of total flavonoids as response value. The results shows that the optimal extraction technology was as follows：ethanol concentration 66％，issue-liquid ratio 16：1（mL/g），extraction time 120min and extraction temperature 70 ℃. Under these conditions，every sample was tested three times，the maximal yield of total flavonoids reached 5.85 mg/g. The total flavonoids the same as model predicated contents which was 5.87mg/g. The technology can be used as the extraction process of total flavonoids from kushui rose. This article also studied the content of quercetin and kaempferol in total flavonoids. Quercetin and kaempferol in a range of good linearity，the correlation coefficient was 0.998 2~0.999 3；precision（RSD） were less than 1.76%；the average recovery was 96.05%~101.15%. This methods was suitable to rapid detection of quercetin and kaempferol in total flavonoids.